摘要
讨论了从牛初乳中分离乳铁蛋白的工艺。结果表明,牛初乳经过离心脱脂得到脱脂乳,加入带羧甲基的弱酸性阳离子交换剂CM-Sepharose Fast Flow在4℃下进行动态吸附,用去离子水将没有吸附上的杂蛋白冲洗掉,然后使用质量分数为1.5%和5%的NaCl溶液进行阶跃洗脱;经过两步阶跃洗脱得到的乳铁蛋白纯度可达95%以上。
The Lactoferrin is the living beings active material in the mammal mamma , and the original mainly talked about the technology of Lactoferrin departs from bovine colostrum ： The bovine colostrum obtains the skimmed milk after being at odds with the community to degrease , and accedes to brining Qiang＇s methyl weak acid cation exchange dose a CM-Sepharose Fast Flow being living to carry on static state below 4℃s adsorbing ; In the way of leave ion water shall not there is , and sundry albumen on the sticking rinses , afterwards employ the NaCl＇s liquor of 1.5% and 5% to carry on the stairs and jumps to wash comeing off ; After two jumps wash to come off over the Lactoferrin pureness attainability 95% of mamma that obtains.
出处
《中国乳品工业》
CAS
北大核心
2005年第8期10-13,共4页
China Dairy Industry
关键词
牛初乳
乳铁蛋白
离子交换层析
bovine colostrum
Lactoferrin
cation-exchange adsoption
