摘要
以宇佐美曲霉AS3.758为发酵菌株、栀子黄废液为原料,以液态发酵方式生产栀子蓝色素,通过正交试验法优选栀子蓝色素的最佳工艺条件。结果为:培养温度为29℃、发酵液pH为6.5、发酵培养时间为36h,发酵结束后经水解、过滤,滤液中加入谷氨酸钠,反应后得栀子蓝色素液,转化率达98.43%。色素液经D301离子交换树脂吸附,再用1.0molL盐酸洗脱,洗脱液经低温干燥得到的栀子蓝色素,色价E1%1cm(590nm)达55.6。
The fermentation and purification technology of gardenia blue pigment were studied in this paper, The results showed that the better conditions were culture temperature 29℃ ,fermentation liquid pH6.5 and fermentation period 36 hours, The culture liquid was hydrolyzed and filtrated, and reacted with glutamic acid, and Gardenia blue was made, the conversion rate is 98.43%, We used D301 ion exchange resin for purifying Gardenia blue pigment , and 1.0mol/L HC1 as washout solvent, the washout liquid was dried ,we got the more pure Gardenia blue pigment （E1cm^1% 590nm≥ 55.6）.
出处
《生物技术》
CAS
CSCD
2005年第4期61-63,共3页
Biotechnology
基金
江西省自然科学基金项目资助(0430003)
关键词
栀子蓝色素
液态发酵
转化率
离子交换树脂
Gardenia blue
liquid fermentation
transformation rate
ion exchange resin
