摘要
采用黑曲霉发酵产β-葡萄糖苷酶,再用戊二醛交联,海藻酸钠包埋法共固定化酶和菌丝,然后将共固定化酶用于水解栀子苷,再与谷氨酸钠反应制备栀子蓝色素。对共固定化条件进行了优化,优化后的条件为:交联剂戊二醛浓度为0.15%,交联温度为20℃,交联时间为2h。同时还对共固定化酶水解栀子苷的条件进行了优化,优化后的水解条件为:水解温度为50℃,水解时间为6h,水解pH为5.0。水解后的转化液与谷氨酸钠反应得到栀子蓝色素溶液,经大孔树脂HPD300吸附洗脱,洗脱液经真空减压浓缩、干燥后得栀子蓝色素粉末,色价E590nm1%为120。共固定化酶水解栀子苷制备栀子蓝色素的工艺与传统方法相比具有成本低、环境友好、易于工业化放大的特点。
Generated β-glucosidase by fermenting Aspergillus niger,and co-immobilized β-glucosidase and mycelium by cross-linking with glutaraldehyde and embedding with sodium alginate,then hydrolyzed geniposide with co-immobilized enzyme,preparated gardenia blue pigment by hydrolysate reacting with sodium glutamate.Optimized co-immobilizing conditions are：glutaraldehyde concentration is 0.15%,cross-linking temperature is 20 ℃,cross-linking time is 2 h.Optimized hydrolyzing conditions are：hydrolyzing temperature is 50 ℃,hydrolyzing time is 6 h,and hydrolyzing pH is 5.0.Produced gardenia blue pigment by hydrolysate reacting with sodium glutamate,then was adsorbed by HPD300 macroporous,then eluted by ethanol,eluate was concentrated by low pressure,and drying,then got gardenia blue pigment powder,it＇s color value E 590 nm 1% is 120.The method of preparation of gardenia blue pigment by geniposide hydrolyzing with co-immobilized enzyme is low cost,environmentally friend,easy-to-industrialization,it is better than traditional method.
出处
《食品科技》
CAS
北大核心
2013年第2期229-233,共5页
Food Science and Technology
基金
国家自然科学基金项目(30960046)
关键词
共固定化酶
栀子苷
栀子蓝色素
co-immobilized enzyme
geniposide
gardenia blue pigment
