摘要
本研究针对ELISA法OD值易因板与板之间以及实验的天与天的不同而变化的问题,以间接法为例,从反应原理的角度,即用抗原-抗体反应和酶反应动力学公式,推导出校正板间误差的数学公式,并用实验对其进行了验证。
We report the establishment of a rectify formula for ELISA to proffread and correct the OD-value difference caused by different experiment days and the varity of plates.The formula was based on the theory of ELISA reactions,it had been also tested and verified by several experiments.
出处
《寄生虫与医学昆虫学报》
CAS
1995年第3期140-145,共6页
Acta Parasitologica et Medica Entomologica Sinica