摘要
目的 :分别建立检测抗六种动物免疫球蛋白 (Ig)抗体的ELISAs法 ,了解这些抗体在人群中的发生率。 方法 :用DEAE 纤维素抽提和 33%饱和度硫酸铵沉淀法 ,分别提取绵羊、马、猪、兔、小鼠、牛六种动物血清中富含IgG组分 ,建立ELISAs法 ,对 5 0 0名健康体检者和 14 4例系统性红斑狼疮 (SLE)、类风湿性关节炎 (RA)及肾疾病患者血中的六种抗动物Ig抗体进行检测。 结果 :5 0 0名健康体检者血清中抗绵羊、抗马、抗猪、抗兔、抗小鼠、抗牛Ig抗体阳性率分别为 2 .0 %、2 .4 %、2 .8%、0 .8%、2 .6 %和 1.2 % ;94例SLE患者抗六种动物Ig抗体阳性率依次为 2 .1%、4 .3%、1.1%、0、0和 2 .1% ;2 4例RA患者阳性率依次为 4 .2 %、0、0、4 .2 %、4 .2 %和 4 .2 % ;2 6例肾疾病患者阳性率依次为 3.8%、0、0、15 .4 %、7.7%和 0。 结论 :健康人和患者中均存在人抗动物Ig抗体 ,应注意此类抗体在临床生物学治疗和实验分析中的干扰作用。
Objectives:To investigate the incidence rates of human anti animal immunoglobulin (HAAIg) in normal subjects and certain patients with SLE, RA and nephropathy. Methods: Using salting out and DEAE cellulose chromatography, six HAAIgs in human serum were detected by ELISA with six IgG rich fractions prepared from sheep, horse, pig, rabbit, mouse and cattle serum respectively. Results: The positive percentages of anti sheep,anti horse, anti pig,anti rabbit,anti mouse and anti cattle Ig antibodies were 2.0%,2.4%,2.8%,0.8%,2.6% and 1.2%, respectively in 500 normal subjects; while 2.1%,4.3%,1.1%,0,0 and 2.1% in 94 patients with SLE; 4.2%,0,0,4.2%,4.2% and 4.2% in 24 patients with RA; 3.8%,0,0,15.4%,7.7% and 0 in 26 patients with nephropathy. Conclusions:HAAIgs exist in sera in normal subjects and patients of SLE,RA and nephropathy. It should be seriously considered that HAAIgs may interfere with the clinical biological treatment and immunological assays.
出处
《医学研究生学报》
CAS
2003年第2期88-90,共3页
Journal of Medical Postgraduates
