摘要
用<sup>3</sup>H脱氧胸腺嘧啶核苷（<sup>3</sup>H-TdR）参入DNA合成的生化学方法和癌细胞形态学方法观察到,不同方法处理腹水型(H<sub>22</sub>)肝癌细胞,其杀伤率,A组（912-HpD-laser）为74.4％;B组（HpD-912）为59.7％;C组（laser）为20.4％;D组（912）为27.0％;E组为0％作为对照。A组与其它组相比,杀伤作用显著增强（P均【0.01）。又通过测定脂质过氧化代谢产物Schiff碱的量观察各组对癌细胞的杀伤作用。Schiff碱有荧光特性,荧光强度越大,Schiff碱含量越高,癌细胞死亡数越多,上述5组的荧光强度测定结果分别是84.6,41.6,45.1,61.1和35.2mm。A组显著高于其它各组（P均【0.01）。进一步用化学发光法证明,912-激光-血卟啉衍生物杀伤癌细胞过程中有过氧化氢产生。上述结果提示,912-激光-血卟啉衍生物杀伤癌细胞的作用与活性氧的生成及其介导的脂质过氧化有密切关系。
We observed that aidiron (912), which extracted from earthworm, had a strong killing effects on mice ascitic hepatoma cells (H22) in vitro by biochemical synthesis of DNA mesured with the incorporation of 3H-TdR and morphological method of the tumor cells. Different methods had different degrees for killing the tumor cells, in Group A (912-HpD-Laser), the percentage of the tumor cells killed was 74.4; Group B (HpD-Laser) was 59.7; Group C(Laser) was 20.4; Group D (912) was 27.0 and Group E (H22) as a control (0%). The results indicate that killing effects of hematoporphyrin dervative-laser combining with (912) were greats trong, compared to Group B, C and D (P<0.0l). We also observed the killing effects of each groups mentioned above on H22 tumor cells by measuring the amounts of the Schiff bases. The more intensive the fluorescence and higher the amounts of the Schiff bases were, the more tumor cells were killed. The fluorescence intensity of Group A was 84.6; Group B was 41.6; Group C was 45.1; Group D was 61.1 and Group E was 35.2mm respectively, Group A was higher (P<0.01).Furthermore by the chemiluminescence (CL) analysis we also discovered that during the procedure of the killing tumor cells with laser-HpD-912 there was the generation of hydrogen peroxide. These results of the determination show that the killing effects of laser-HpD-912 on H22 tumor cells were closely related with the production of reactive oxygenic species and which induced lipid peroxidation.
出处
《第四军医大学学报》
1989年第6期376-379,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金No.86030835
关键词
激光
血卟啉类
癌地龙
癌细胞
杀伤
hematoporphyrins
laser
aidiron (912)
tumor cell (H_(22))
free radicals
