摘要
单纯疱疹病毒性角膜炎是临床上常见病多发病,占角膜病首位,但目前仍缺乏理想的病因诊断方法。过去有细胞学检查,病毒分离和免疫荧光的检测等。
Monoclonal antibodies against HSV-l were used in immunoperoxidase technique to detect HSV-1 antigens in epithelial cells of cornea and neurons of trigeminal ganglion. We observed the experimental herpes simplex keratitis on 16 eyes of 8 rabbits and 8 eyes of 4 mice. The test suggests that (1) The method can show HSV-1 antigens in epithelial cells of cornea more sensitively and specifically than that of prior studies. It may be used for rapid diagnosis of HSV infection clinically. (2) It may be used to localize HSV-l antigens in tissues by improving the method. (3) Brown particles can be easily seen by counterstai ning with methylgreen.
出处
《眼科研究》
CSCD
1989年第3期132-134,共3页
Chinese Ophthalmic Research
