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用单克隆抗体免疫酶组织化学技术检测单纯疱疹病毒 被引量:1

Diagnosis of Herpes Simplex Virus Infection with Monoclonal Antibody Immunoenzy matic Histochemical Technique
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摘要 单纯疱疹病毒(HSV)性疾病是人类最常见传染性疾病之一。危害面广,引起眼、口、呼吸道、胃肠道、唇、皮肤、泌尿生殖系、神经系感染,亦可致胎儿和新生儿的感染。近年来激素和免疫抑制剂使用增加。 The anti-HSV-Mc Ab immunoenzymatic histochemical technique was used for the diagnosis of 40 cases of herpes simplex keratitis of different types, 3 cases of genital herpes and 2 cases oral herpes, with positive rate of 93.3%(42/45). All normal tissues gave negative reaction. Compared with the positive rate 96.0% of indirect immunofluorescence technique by anti-HSV-Mc Ab in our laboratory, there is no significant difference (p>0.05). It was about 10.0% higher than that of Zhang's report, in which animal antiserum immunoenzymatic staining was used for the diagnosis of herpes simplex keratitis. And the experimental conditions have been optimized. The method is considered to be specific, sensitive, quick, simple and easy routine laboratories.
出处 《眼科研究》 CSCD 1989年第1期18-21,共4页 Chinese Ophthalmic Research
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