摘要
应用杂交瘤技术，以２种抗原分别免疫ＢＡＬＢ／Ｃ小鼠。从３次细胞融合中，筛选出４株分泌高滴度、高特异性抗Ａ１血型物质单克隆抗体的杂交瘤细胞系。经多种免疫化学试验证明：其中Ｂ５２３－Ｈ１０、Ｄ２４３－Ｈ９２株杂交瘤扩大培养上清液仅凝集Ａ１红细胞，而不凝集Ａ２、Ｂ、Ｏ型红血胞。它们结合部位的结构互补于：人工接上脂肪链的Ａ－活性三糖。从而表明该２株单抗为首次获得的抗Ａ１亚型红细胞单克隆抗体。不仅为研究Ａ１亚型血本质提供了极好的材料，也为医学临床提供Ａ１亚型血分型试剂准备了条件。
Using hybridoma technique,We prepared a group of byhridomas by different immunization procedures.They secreted high level and high specific monoclonal antibodies to A1blood substances.It was demonstrated that the tissue culture superatant of two of them(D234-H9 and B523-H10)could only agglutinate human A1-type erythrocytes specifically,but could not agglutinate A2,B and O type erythrocytes by various immunochemical assays.The complemental structure of their combining site was most complementary to the synthetic A blood group nonreducing trisaccharides glycosidically linked to -O(CH2)8 COOH.The results shaw that this is the first report of two monoclonal antibodies to human A1-type erythrocytes which will offer excellent material for the study of the nature of A,subgroup blood.They can also offer the grouping reagents for A1 subgroup blood in clinical medicine.
出处
《安徽医科大学学报》
CAS
1995年第3期165-168,共4页
Acta Universitatis Medicinalis Anhui
基金
国家教委优秀青年教师基金
第三世界科学院基金
国家自然科学基金
关键词
杂交瘤
单克隆抗体
血型糖蛋白类
红细胞
hybridoma
monoclonal antibody
antibody combining site
glycophorins
erythrocyte
