摘要
采用免疫组化染色检测肝癌组织及癌旁肝组织ＨＢｓＡｇ、ＨＢｃＡｇ以及Ｐ５３蛋白的表达。结果显示：癌灶组织Ｐ５３蛋白阳性率２６．７％（１２／４５），癌旁肝组织无１例Ｐ５３蛋白阳性；Ｐ５３蛋白表达与ＨＢｓＡｇ无明显关系，而与肝癌发生年龄及癌细胞分化程度有关。提示：ｐ５３基因突变与ＨＢＶ相关肝癌的发生有一定关系，而多数肝癌组织并无Ｐ５３基因突变又进一步提示肝癌发生尚涉及其它复杂机制；此外Ｐ５３蛋白的免疫组化检测对肝癌预后的判断有一定价值。
o elucidate
the relationship of p53 gene alterations and hepatocellular
carcinoma(HCC),and toanalyse the association between hepatitis B
virus( HBV)and p53 gene mutation,we detected HBsAg,HBcAg and p53
protein in HCC tissues and nontumorous liver tissues by
immunohistochemical method。The results showed that of the 45
cases,12 cases showed nuclear staining of p53 protein, and no
anystaining in nontumorous tissues.A significant association was
observed between mutant p53 expressionand the patient’s age at
diagnosis or the degrees of HCC cell differentiation。However p53
expression wasno correlation to sex and HBsAg expression, Our results
suggested that p53 altertions played a significantrole in
HBV-associated hepatocarcinogenesis。In addition,the detection of
mutant p53 protein may bevaluable for evaluating the prognosis of
HCC。
关键词
肝细胞癌
P53蛋白
免疫组化
HBV
Hepatocellular carcinomaa p53 protein
Immunohistochemistry
