摘要
利用血清学ＥＬＩＳＡ方法和ＰＣＲ技术对１６９例输血后肝炎（ＰＴＨ）患者病因学进行了研究。结果表明，ＰＴＨ的主要致病原为ＨＣＶ（９８．２２％），其中部分患者（２４／１６９）同时合并有ＨＢＶ感染，３例ＰＴＨ患者病因仍不明确。ＨＣＶ感染所致ＰＴＨ患者抗ＨＣＶ检出时间为ＰＴＨ症状出现后７ｄ～１年，平均为５４．６２ｄ；ＨＣＶＲＮＡ在感染早期（６～２０ｄ）即可检出。对８４例ＰＴＨ患者１．５年～３年的随访观察结果表明，抗ＨＣＶ可长时间持续存在，而ＨＣＶ病毒血症可有三种表现形式；一过性、持续性和间歇性病毒血症。ＨＣＶ／ＨＢＶ重叠感染者，ＨＣＶＲＮＡ检出率为１００％（２４／２４），明显高于ＨＢＶＤＮＡ检出率（１６．６７％，Ｐ＜０．０１）。４例ＨＢＶＤＮＡ阳性者，ＨＢＶＤＮＡ和ＨＣＶＲＮＡ交替检出，似乎提示重叠感染时，ＨＣＶ可抑制ＨＢＶ复制，或两者之间相互干扰，对此应进一步研究证实。
169 patients of post-transsion
hepatitis(PTH)were investigated for etiology with serological and PCR
methods. Our result showed that HCV was the major causative agent of
PTH in this studv。Some patients( 24/169 )were HBV markers positive
too. Three patients were negative for all viral markers。Anti-HCV was
detectable from 7 days to one year later after onseting of clinical
symptoms,average time 54. 62 days. HCV RNA could be detected in early
time of HCV infection(6 to 20 days later), average time 8. 72 days. A
follow-up study of 84 PTH patients caused by HCV infection showed
that all patients remained anti-HCV positive during the observation
period(1.5～3years),but their HCV viremia had three
forms:transient,persistent or interminant.The positive rate of HCV
RNA higher than that of HBV DNA in patients with HCVlHBV
superinfection(100%, 16. 67%,P<0.01). All four HBV DNA positive
patients were HBV DNA and HCV RNA positive alternatively during t he
observation. This re- sult suggested that there was a suppression of
HBV replication by HCV infection or the interaction of HBV and HCV
during superinfection。
基金
国家八.五科技攻关重点资助
