摘要
本研究采用大鼠黄体细胞体外孵育技术，观察了缩血管肽内皮素－１（ＥＴ－１）对黄体细胞孕酮分泌的影响及其与细胞内信使ｃＡＭＰ的相互关系。结果表明，１０－６ｍｏｌ／Ｌ的ＥＴ－ｌ可显著抑制黄体细胞的基础孕酮分泌和ｈＣＧ诱导的孕酮分泌；外源性ｃＡＭＰ和腺苷酸环化酶激动剂Ｆｏｒｓｋｏｌｉｎ则可促进黄体细胞分泌孕酮；当ｃＡＭＰ或Ｆｏｎｓｋｏｌｉｎ与ＥＴ－１共同作用时，前者可完全反转ＥＴ－１对黄体细胞的抑制作用，孕酮分泌恢复到对照组水平。提示Ｅｌ－１可能是一种卵巢局部调节因子，通过抑制黄体细胞内的腺苷酸环化酶，减少ｃＡＭＰ的生成和释放，进而抑制黄体细胞的孕酮分泌。
Objective :To determine the effect of endothelin-l on the progesterone( P)ecretion by rat luteal cells and its relation with cAMP. Design :In vitro experimental study. Setting:Laboratory in Shanghai lnstitute of Planned Parenthood Research. Methods:Preparation of luteal cells suspension from Sprague-Dawley rat ovaries and incubation in KR-BGA1 solution,Samples of incubation medium were collected and stored at-20℃for measurement of Pand cAMP with radioimmunoassay. Results:Endothelin-l at a concentration of l0-6mol/L significantly reduced the basal and hCG inducedP secretion about 20%and29%respectively (P<0. 0l ).xogenous cAMP significantly increased the p se-cretion to about 22%of the control group(P<0.05).When added to the endothelin-1 treated medium theP secretion returned to the control level. Forskolin stimulated the P secretion to 2.l times higher than thecontrol (P<0. 0l ),and when added to the endothelin-l culture system,the P secretion increased to a levelhigher than the control (P<0.01).The cAMP concentration in the luteal cell mediuM was significantly re-duced(P<0.05)when culltured with endothelin-l. Conelusion :The study suggested that endothelin-1 may be an intra- ovarian regulatory factor which in-hibits progesterone secretion from rat luteal cells by suppressing the adenyl cyclase activity and reducingcAMP accumulation .
出处
《生殖医学杂志》
CAS
1995年第2期88-91,共4页
Journal of Reproductive Medicine
基金
国家自然科学基金及江西省自然科学基金
关键词
内皮素
CAMP
孕酮
黄体
Endothelin cAMP Progesterone Luteal cels Rats.
