摘要
我们采用黄体细胞孵育的方法，观察了内皮素－１对离体黄体细胞孕酮生成的影响。发现内皮素－１能抑制黄体细胞孕酮的基础分泌及人绒毛膜促性腺激素（ＨＣＧ）刺激下的孕酮分泌。内皮素－１加入孵育的黄体细胞悬液１５分钟后，即发生明显地抑制孕酮分泌作用（Ｐ＜０．０１）。人内皮素－１的抗血清能反转内皮素－１的上述作用。实验结果提示，内皮素－１可能为一种卵巢内调节肽，它通过抑制黄体细胞的孕酮生成。
The effect of endothelin- 1 on progesterone production by incubated rat luteal cells wasstudied in the present investigation, Endothelin-1 could inhibit the basic progesteroneproduction(10-7mol / L, p<0.05) and the progesterone secretion induced by hCG(10-9mol / L,P<0.05)from rat luteal cells. The beginning time is 15 min.The effect of endothelin-1 could be prevented speciallyby endothelin-1 antiserum.This study shows that endothelin-1 combines with its special binding sites onthe luteal cells, which leads to the decrease of progesterone production and suggests that endothelin-l isan intra-ovarian regulatory factor and is involved in the process ofcorpus luteum involution.
出处
《生殖医学杂志》
CAS
1994年第3期159-162,共4页
Journal of Reproductive Medicine
基金
国家及江西省自然科学基金
关键词
内皮素
黄体
孕酮
Endothelin Luteal Progesterone
