摘要
采用小鼠Ⅲ度烧伤模型,观察了溶葡萄球菌酶对烧伤后腹腔巨噬细胞吞噬、消化鸡红细胞能力,化学发光强度以及全身碳末廓清功能的影响,旨在探讨溶葡萄球菌酶改善机体抗感染能力的作用。结果表明:烧伤后小鼠腹腔巨噬细胞吞噬消化能力和化学发光强度均明显降低;碳末廓清功能明显受损。给予溶葡萄球菌酶后,小鼠腹腔巨噬细胞吞噬和消化鸡红细胞能力明显改善,化学发光强度增强,并超过烧伤组和正常对照组。同时溶葡萄球菌酶还可增强全身碳末廓清功能,其 a 值可达1.63×10^(-2),显著高于烧伤组(8.13×10^(-4))和正常对照组(2.76×10^(-3))。提示溶葡萄球菌酶具有增强吞噬细胞功能,进而改善机体抗感染能力的作用。
The ability of Lysostaphin to prevent immunosuppresion of phagocyte was assessed in burn mice.A full thickness burn covering 20% of TBSA was created on back of mouse.Lysostaphin was given intraperitoneally(400μg/kg/day)for 3 days in treatment group.The results showed that Lysostaphin treatment could restore the de- pression of phagocytosis of chick RBC by peritoneal macrophages.In the group treated by Lysostaphin,peritoneal macrophage chemiluminescence expressed much greater peak value than that in both burn and normal groups.The a-ralue,which is a criterion of car- bon clearance,was 1.63×10^(-2)in lysostaphin treated mice,while that in burn and nov- mal mice were 8.13×10^(-4)and 2.76×10^(-3),respectively.Results of the study indicated that Lysostaphin was effective in improving phagocytosis by phagocytes.
