摘要
以一株能转化鹅去氧胆酸（ｃｈｅｎｏｄｅｏｘｙｃｈｏｌｉｃａｃｉｄ，ＣＤＣＡ）为熊去氧胆酸（ｕｒｓｏｄｅｏｘｙｃｈｏｌｉｃａｃｉｄ，ＵＤＣＡ）的厌氧菌－ＣｌｏｓｔｒｉｄｉｕｍｐｅｒｆｒｉｎｇｅｎｓＴｙｐｅＡＨＳ－１０（产气英膜梭菌ＨＳ－１０）作为试验菌，进行细胞固定化，研究不同的吸附和包埋材料制备的固定化细胞对ＵＤＣＡ生物转化的影响，探索提高底物浓度、菌种底物耐受度及转化率的可能性。实验结果表明，海藻酸钙、多孔陶瓷和蛭石都是较为理想的固定化材料，特别是先将菌体在含１．２ｍｍｏｌ／Ｌ的ＲＣＭ（ｒｅｉｎｆｏｒｃｅｄｃｌｏｓｔｒｉｄｉｕｍｍｅｄｉｕｍ）培养基中进行＂预培养＂，然后再经２％的海藻酸钙固定化后能显著提高底物耐受度，当底物浓底高达１．０ｍｍｏｌ／Ｌ和２．０ｍｍｏｌ／Ｌ时，最大转化率分别为４０％和２０％
Clostridium perfringens HS-10 was immobilized to study the biotransformation ofchenodeoxycholic acid(CDCA) to ursodeoxycholic acid in order to increase the substrateconcentration ,the anaerobe tolarence to substrate and the bioconversion rate, the iniinobilzedcells were made with ditferent adsorbing and embedding materials. It was found tbat calciumalginate, porous eramic and vermiculite were perfect immobilizing materials if the anaerobe HS -10 was 'pre-cultured'in RCM medium containing 1.2mmol/L CDCA, the tolerence tosubstrate could be remarkably mised when the bacterial cells were embedded with 2% calciumalginate.The greatest conversion rates were 40% and 20% with substrate concentiations up to1.0mmol/L and 2.0 mmol/L.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1994年第3期94-98,共5页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国家自然科学基金
暨南大学青年基金资助项目
关键词
固定化细胞
熊去氧胆酸
生物转化
anaerobe, immobilized cell,ursodeoxycholic acid,biotransformation
