摘要
在陕西杨陵地区栽培的千金子花叶毒林上分离到一种病毒,经汁液磨擦接种三生烟及心叶烟产生环斑症状。其TIP为60℃,DEP为10^(-2)~10^(-3),L则为4~5天。能通过种子传播但不能通过蚜虫传播。该病毒与TRSV抗血清有明显的沉淀反应。在电镜下观察到直径30nm的球状粒子。该病毒被鉴定为TRSV。
A virus isolated from severely mosaic moleplant (Euphorbia lathyrus) in Yangling areas Shanxi province can infect Nicotiana tabacum CV. Samsun and N. glutinosa forming ringed sports by sap inoculation. The thermal inactivation point of virus is 60℃, the dilution end point is 10^(-2)-10^(-3), and the longevity in vitro is 4-5 days. The virus can be transmitted by seed but not aphid. The virus reacts on antiserum of tobacco ringsport virus (TRSV) to form precipitation. Under EM, the virus presents 30mm in diameter. Mentioned above results the virus is identified as TRSV.
出处
《西南农业大学学报(自然科学版)》
CSCD
1989年第3期261-263,共3页
Journal of Southwest Agricultural University
关键词
千金子
花叶病
烟草
环斑病毒
tobacco ringspot virus
certification
mosaic
Euphorbia lathyris.