摘要
以番木瓜环斑病毒(PRV-SM株)基因组RNA的cDNA为模板,通过聚合酶链式反应(PCR)合成和改造了病毒外壳蛋白基因。将该基因克隆于pBluescript KS以后,完成了其全序列分析。与PRV-P和W株系比较,其核苷酸序列同源性分别为89.4％和88.7％;由核苷酸序列所推导出的氨基酸序列同源性均为94.4％。SM株与P、W株间的同源性低于P株与W株间的同源性。Western-blot分析表明,所克隆的病毒外壳蛋白基因在大肠杆菌中能获得正常表达产物,它具有与天然外壳蛋白同样的大小及相同的血清学反应。
Using genomic RNA of papaya ringspot virus ( SM strain ) as temp-latc, cDNA was reverse-transcriptedj and from which virai coat protein gene was synthesized and modified by polymerase chain reaction(PCR). After cloning into plasmid pBluescript KS, the total nucleotide sequen-ce of the coat protein gene was determined. When compared with the equivalent regions of the P and W strains of PRV, the homology of nucleatide sequence were 89.4% and 88.7% respectively5 and amino acid sequences 94.4% in both cases. These data suggest that the homology between PRV-SM and P or W strain is much less than that between P strain and W strain. The PRV coat protein gene was expressed cor-rectly in E.coli by Western-blot analysis. The size and the serologica-lly specific reaction of the expressed products are the same as the .au-thentic virai coat protein.
出处
《病毒学报》
CAS
CSCD
北大核心
1993年第1期78-84,共7页
Chinese Journal of Virology
基金
国家"八.五"攻关课题
关键词
番木瓜
环斑病毒
外壳蛋白基因
Papaya ringspot virus, Coat protein gene, Sequence analysis, Gene expression
