摘要
从继代3年以上的胡萝卜(Daucus carota var.sativa Dc.)非胚性愈伤组织酶解分离出大量成活的原生质体,在 DPD、V—KM 和 C81V 培养基中液体浅层培养,获得微愈伤组织后,在固体 N_6和 MS 培养基上分化培养,获得再生小植株。证明长期继代培养的胡萝卜愈伤组织仍具有再生植株的能力。
This paper deals with the culture of protoplasts of carrot(Daucuscarota Var.satire DC)and regeneration of plantlets.Numerous viableprotoplasts isolated enzymatically from friable non—embryogenic calliwere subcultured for more than three years on Murashige and Skoog'smedium(MS)supplemented with 1.5—2.0 mg/l 2.4-D and 0.2—0.5 mgkinetin or zeatin.They were cultred in thin liquid layer of DPD,C81 Vand V—KM media,plus 1.5 mg/l 2.4—D,0.5 mg/l zeatin,500 mg/l yeastextract(YE)or casein hydrolysate(CH),200mg/l L-glutamine.The pro-toplasts underwent sustained divisions and formed protocolonies,Aftertransferring them onto MS and N_6 solid regeneration media with differenthormones concentration and combination the calli grew further and rege-nerated plantlets via embryogenesis or organogenesis.
出处
《西南农业大学学报（自然科学版）》
CSCD
1989年第1期102-104,共3页
Journal of Southwest Agricultural University
关键词
胡萝卜
原生质体培养
再生
carrot
Protoplast culture
regeneration
