摘要
在以N_6为基本培养基,附加水解乳蛋白800mg/l、蔗糖3%、椰乳4%、2,4—D1.0～2.5mg/l、NAA0.2～0.8mg/l、ABA 0.01mgl、6—BA 0.5mg/l的液体培养基中,对谷子(Setaria italica(L.)Beauv)幼穗起始的愈伤组织细胞进行悬浮培养,通过体细胞胚胎发生获得大量完整的小植株。研究还证明,低浓度的ABA可促进胚状体的形成,一定量的活性炭对促进胚状体发育成熟和出苗成株也有明显效果。
Young infloresence of Foxtail Millet (Setaria italica(L. ) Beauv.), cultured in N_6 medium, produce a pale—yellow vigorous callus. They were cultured in liquid N_6 media supplemented with2.4—dichloropheoxy acetic acid ( 2.4—D ) 1.0～2.5mg/1.6—benzylaminopurine(6—BA) 0.5mg/l, naphthalene acetic (NAA)0.5mg/l and abscisin acid(ABA) 0.01mg/l, lactalbumin hydrolsate( LH ) 800mg/l, coconut milk (CM, 4%, v/v), sucrose 3% on a gyrotory shaker to establish embryogenic suspension cultures composing of large, elongated and highly vacuolated nonembryogenic cells and small, contained rich cytoplasm embryogenic cells. The ratio of the two component cells to a certain extent can be manipulated by varying the duration of each sub—culture and the volume of the inoculum used during sub—culture.
The embrygenic cells underwent rapid and continuous divisions to form larg embryogenic microcallus and further to produce proembry oids. These microcalli, transferred onto agar differention media plused activated charcoal and ABA, prodttced many mature embryoids and high—efficiently formed regenerated plantlets via germination of embryoids. The result indicated that a little of ABA might be a promoter onembryogenesis and the charcoal also efficiently promoted the formation of embryoids and their germination to regenerated rooted plantlet. This study laid the basis of protoplast ctilture, somatic hybridization, transfermation of cells and selection for resistant mutant of foxtail millet.
出处
《西南农业大学学报（自然科学版）》
CSCD
1990年第4期379-383,共5页
Journal of Southwest Agricultural University
关键词
谷子
悬浮培养
体细胞
胚胎发生
Setaria italica (L.) Beauv., Suspension Culture
somatic embryogenesis/plant regeneration
