摘要
目的为了获得浙江省汉坦病毒基因组更为详尽的资料,研究汉坦病毒的进化状况及变异程度,为疫苗病毒株的选择使用提供科学依据。方法本实验室利用RT-PCR方法扩增ZT71株S基因片段并克隆入质粒载体,进行核苷酸序列测定及分析。结果ZT71株S基因由1754个核苷酸组成,只有一个开放读码框架,共编码429个氨基酸。与HTN型病毒(76-118)的核苷酸和氨基酸同源性分别为72.0%和82.6%,与SEO型病毒(SR-11、R22、Guo3、8610)核苷酸和氨基酸同源性分别为88.4%—96.5%和98.1%—99.0%,与其它型汉坦病毒的同源都很低。结论表明此分离的病毒为SEO型汉坦病毒,为进一步研究其进化和变异提供了有利条件。
The S segment cDNA of hantavirus ZT71 strain was obstained by reverse transcription and polymerse chain reaction,subsequently cloned into pGEM-T vetor.The sequence of positive recombinants was determined by the method of didioxy chain termination,which revealed that the S segement was 1754 nucleotide in length with a open reading frame encoding a protein of 429 amino acids.The comparison with HTN type(76-118 strain)indicated that there was 72.0% of homologg at the nucleotide level and 82.6% of homology at the amino acid level.Comparison with SEO type(SR-11,R22,Guo3,8610 strains)showed 88.4%-96.5% homology at the nucleotide level,98.1%-99.0% homology at the amino acid level.The results of nucleotide and amino acid comparisons at molecular level indicated that ZT71 strain is SEO Hantanvirus.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2005年第7期570-573,共4页
Chinese Journal of Zoonoses
关键词
汉坦病毒
ZTT1株
S片段
序列分析
<Keyword>Hantanvirus
ZT71 strain
S genome segment
sequence analysis
