摘要
目的研究深圳市引起肾综合征出血热(HFRS)的汉坦病毒主要型别及分子特征,为该市HFRS的防治提供依据。方法收集各医院送检HFRS患者急性期血清标本,提取病毒RNA,采用实时荧光PCR方法进行分型检测;逆转录-套式PCR扩增基因组M片段G2区和S片段,测序后进行同源性比对和系统进化树分析。结果共收集深圳市HFRS IgM抗体阳性血清标本123例,其中男性97例,女性26例。病例多发于青壮年男性。全年均有病例,春季及夏季初期为高发时段。荧光PCR结果显示汉坦病毒阳性标本为63例,阳性率为68.5%,其中SEO型59例,HTN型4例,且HTN型均为男性。序列比对和系统进化树分析发现,深圳地区流行的SEO型汉坦病毒核苷酸序列的差异相对较小,除2例SEOV S3亚型外,大多数为SEOV S2亚型。HTN型汉坦病毒的变异率较高,其中H5亚型和H8亚型各有2例。结论深圳市HFRS患者当前感染HV仍然以SEOV为主,尤其是S2型,与广州市部分毒株亲缘关系较近,与L99、Z37等疫苗株有较高同源性。首次发现S3亚型在深圳市的存在,同时仍然有HTNV病例的输入。
Objective To study the genotype and molecular characteristics of Hantavirus causing hemorrhagic fever with renal syndrome(HFRS)in Shenzhen so as to provide basis for the prevention and control.Methods The serum samples of HFRS patients at acute stage were collected from hospitals,and viral RNA from the sera was extracted as a template for typing detection by real-time fluorescent PCR.The nucleotide sequences of M fragment(G2 segment)and S fragment were amplified by reverse transcription-nested polymerase chain reaction(RT-nested PCR).The PCR products were then sequenced and homology and phylogenetic tree analysis were conducted.Results In a total of 123 HV IgM antibody positive serum samples were collected in Shenzhen from 2015 to 2023,including 97 males and 26 females.Most of patients were young and middle-aged men.Cases were found throughout the year,with high incidence in spring and early summer.The result of fluorescence PCR showed that among 92 clinical specimens,63 cases(68.5%)were tested positive for hantavirus genotypes,59 cases(93.7% of positives)were identified as SEOV and 4 cases(6.3% of positives)as HTNV.Notably,all HTNV-positive patients were male.Analysis on nucleotide homology and phylogenetic tree showed that the difference in nucleotide sequences among SEO viruses in Shenzhen was small,and most of them were S2 subtype except for two cases of S3 subtype.The mutation rate of HTN viruses was high,with two cases of H5 subtype and two cases of H8 subtype.Conclusions The major genotype of hantavirus causing HFRS in Shenzhen is SEO genotype,especially S2 subtype,which is closely related to some strains in Guangzhou,and has high homology with L99,Z37 and other vaccine strains.The existence of S3 subtype cases was detected for the first time in Shenzhen,while some cases of HTNV were still imported.
作者
吴丽娜
李玥
黄亚兰
吴春利
黄达娜
阳帆
Wu Lina;Li Yue;Huang Yalan;Wu Chunli;Huang Dana;Yang Fan(College of Public Health,Southern Medical University,Guangzhou 510515,China;Institute of Pathogenic Biology,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China)
出处
《中华实验和临床病毒学杂志》
2025年第3期324-332,共9页
Chinese Journal of Experimental and Clinical Virology
基金
病毒学国家重点实验室开放研究基金项目(2023KF003)
深圳市科创委基础研究面上项目(JCYJ20220530140600001)
深圳市医疗卫生三名工程项目(SZSM202211023)。
关键词
肾综合征出血热
汉坦病毒
基因分型
序列分析
系统进化树
Hemorrhagic fever with renal syndrome
Hantavirus
Genotype
Sequence analysis
Phylogenetic tree
