摘要
用成年雄性Ｗｉｓｔａｒ大鼠，分为溃疡组和对照组。用免疫电镜包埋后免疫金间接法标记ＡＣＴＨ细胞，研究胃溃疡自愈过程中垂体远侧部ＡＣＴＨ细胞的超微结构变化。结果显示，在实验性胃溃疡第４ｄ，一些ＡＣＴＨ细胞含少量很小的分泌颗粒，沿质膜分布。有较多的ＡＣＴＨ细胞胞质出现许多空泡。溃疡第６ｄ，ＡＣＴＨ细胞的高尔基复合体发达，其扁囊膨大部含中等电子密度的内容物，其内可见金颗粒标记。溃疡６～１０ｄ，较多的ＡＣＴＨ细胞含许多分泌颗粒。溃疡第２８ｄ，ＡＣＴＨ细胞的超微结构基本恢复正常。本研究结果表明，在大鼠实验性胃溃疡自愈期间，垂体远侧部ＡＣＴＨ细胞的超微结构显示明显的变化。这些变化主要反映了ＡＣＴＨ细胞合成、贮存及释放激素的活动增强，提示垂体远侧部ＡＣＴＨ细胞参与了溃疡自愈过程的调节。
Adult male Wistar rats were used and divided into experimental gastric ulcer group and normal control group.Electron microscopic postembedding indirect immuno-gold labelling technique was used to show the ACTH cells. Ultrastructural changes of ACTH cells during ulcer healing process were studied in this paper. On the 4th day of ulcer,it was observed that some ACTH cells contained a few small secretory granules which distributed along the plasma membrane,and some ACTH cells showed many vacuoles in cytoplasm,On the 6th day,Golgi complex of the ACTH cells became well-developed.The flat sacs of Golgi complex contained moderate electron-dense material which could be labelled by colloidal gold particles. During the 6th to 10th day,more cells contained many secretory granules. On the 28th day,the ACTH cells showed same ultrastructural features as the normal.The above results indicated that the ACTH cells in the pituitary pars distalis showed obvious ultrastructural changes during healing process of the experimental gastric ulcer,These changes may reflect the synthesis,release and storage of hormone in the ACTH cells became active during ulcer healing.The results suggest that the ACTH cells participate in regulation of ulcer healing.
出处
《解剖学报》
CAS
CSCD
北大核心
1994年第4期403-407,T014,共6页
Acta Anatomica Sinica
关键词
ACTH细胞
胃溃疡
免疫电镜
ACTH cell
Pituitary pars distalis
Experimental gastric ulcer
Immunoelectron microscopy
Rat
