摘要
利用玉米获白×莱1029的未成熟胚诱导的愈伤组织,系统地研究了愈伤组织的改良、悬浮细胞系的建立及其分化。结果表明,获白×莱1029的愈伤组织在高硝态氮的培养基上,经过调节2,4-D的浓度、渗透压和NaCl的浓度,选择得到了黄色、小颗粒状结构、不分泌粘液、快速生长和易于悬浮的松脆愈伤组织:在悬浮培养初期,经过高浓度2,4-D的启动,短时间内先后建立了4个悬浮细胞系,并对其中一个系的生长特性进行了鉴定:采用过渡分化的方法,悬浮细胞系再生了7株完整、健壮的试管苗。文章还对愈伤组织改造中所采用的措施进行了单因子分析,对有关问题进行了讨论。
The whole establishment process of suspension cell lines, including callus induction, callus texture modification, establishment and differentiation of suspension cell lines, was systematically studied with immature embryos from H×L genotype in maize. A kind of golden, non-mucilaginous, grainy texture, friable and fast growing callus systematically studied with immature embryos from H×L genotype which is suitable for suspension cul-ture was obtained through modification of selected type Ⅱ callus from H×L genotype on the modified MS medium with high level of nitratenitrogen cooperated with adjusting 2, 4-D concentration, osmotic pressure, NaCl concentration. So far the four suspension cell lines have been established through several steps, particularly including short-period sus-pension culture of the modified callus in the liquid medium containing high concentration of 2, 4-D to switch on thses cells adapting growth in liquid medium.Seven plantlets have been regenerated by using the method of transition differenti-ation of plated suspension cells on the solid media.This paper has also systematically studied appropriate concentration ranges of sev-eral factors used in the callus modification. Discussion has been made in several aspects.
出处
《作物学报》
CAS
CSCD
北大核心
1994年第2期168-175,共8页
Acta Agronomica Sinica
关键词
玉米
悬浮细胞
组织培养
植株再生
Maize suspension cells, Maize cell culture, Plant regeneratoin.
