摘要
目的观察还原型谷胱甘肽(GSH)对1甲基4苯基1,2,3,6四氢吡啶(MPTP)损伤的小鼠黑质多巴胺神经元的影响及可能的作用机制。方法雄性C57BL小鼠给予还原型谷胱甘肽300mg/(kg·d)腹腔注射连用10天,第6天预防组另于皮下注射MPTP40mg/(kg·d),再应用5天,其间观察小鼠行为学变化;应用免疫细胞化学染色法观察黑质区酪氨酸羟化酶(TH)阳性神经元及活化型caspase3阳性细胞的变化。结果还原型谷胱甘肽可以抑制小鼠的黑质酪氨酸羟化酶阳性神经元减少及活化型caspase3阳性细胞激活。结论还原型谷胱甘肽可能对MPTP损伤的黑质多巴胺神经元凋亡有抗氧化保护作用。
Objective To observe the effect of reduced glutathione on dopamineric neurons in the substantia nigra in mice with a 1 methy 4 phenyl 1,2,3,6 tetrahydropyridine(MPTP) lesion and to study its possible mechanism.Methods C57BL mice were administrated i.h.with MPTP (40mg/kg·d) in 5 days to produce PD mouse model.Reduced glutathione (300mg/kg·d) were given i.p.5 days prior to MPTP in preventive groups and used consecutively for 10 days.Meanwhile,observed the varities of mouse behavior.Immunocytochemistry staining was used to detect Tyrosine hydroxylase (TH) and activation of caspase 3 positive cells.Results Pretreatment with reduced glutathione could prevent the loss of TH positive neurons and decrease the number of activation of caspase 3 positive cells.Conclusion Reduced glutathione may have protective effect on apoptosis and oxidative stress of substantia nigra neurons in MPTP induced PD mouse model.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第3期256-258,F004,共4页
Journal of Harbin Medical University
