摘要
目的研究氯化锂(LiCL)对KM小鼠中脑黑质受损伤的多巴胺能神经元的保护作用。方法利用1-甲基4-苯基1,2,3,6四氢吡啶(MPTP)腹腔注射以损伤小鼠中脑黑质多巴胺(DA)能神经元,再将实验动物分实验组,腹腔注射氯化锂;实验对照组,腹腔注射PBS;和空白对照组(MPTP注射后不再注射其他物质)。动物存活一周后,一部分取其中脑黑质节段,固定、包埋做连续冠状石蜡切片,以免疫组织化学染色方法,显示各组酪氨酸羟化酶(TH)和钙结合蛋白(CB)表达阳性细胞,光镜观察并细胞计数,统计学分析;另一部分取其中脑黑质组织匀浆,行TH、CB的Westernblot测量,用Labworks软件分析处理。结果免疫组织化学染色结果显示:实验组小鼠黑质致密部TH与CB阳性神经元数量显著多于实验对照组;Westernblot免疫印迹结果显示:实验组TH、CB蛋白含量表达水平显著多于实验对照组。结论氯化锂对成年PD小鼠黑质多巴胺能神经元具有保护作用,且这种保护作用可能与细胞内CB表达增加有关。
Objective To study the effects of Lithium Chloride protection on dopamineric neurons injuried in the substantia nigra. Methods MPTP was injected into intraperitoneal to injury the DA neurons in the midbrain nigra of mice. The mice models were divided into the study group （Lithium Chloride group） ,the control group（PBS group） and sham operated group. After varied survival period ,the model mice were sacrificed to get the segment of substantial nigra （SN）,which then were fixed ,embedded and co ronally sectioned continuously. The microsections were processed by immunohistoch.emistry labeling separately to show the DA neurons （tyrosine hydroxylase）and CB-containing neurons（CalbindinD28k）with anti-CB. The labeled cells were counted under microscope and analyzed statistically. （n=6）. Western blot assay was used to examine the protein expression of TH and CB in SN of mice （n=4）. After blotting,the bands on the filter were scanned and analyzed with an image analyzer of Lab works. Results In the study of Lithium Chloride on the DA neurons,the number of TH and CB positive neurons was significantly more than that of the control group（P〈0.01）. Meanwhile,the level of protein expression of TH and CB were also significantly higher （P〈0.05）. Conclusion Lithium Chloride protects Dopaminergic neurons, and that role may be related with the increase of CB expression.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2006年第6期652-654,共3页
Journal of Apoplexy and Nervous Diseases
基金
教育部科学技术研究重点项目(No.204055)
