摘要
应用胎儿原代小肠细胞，研究黄嘌呤／黄嘌呤氧化酶（Ｘ／ＸＯ）体系所产生的活性氧对小肠上皮细胞的损伤作用及药物防治效果。结果：加入Ｘ／ＸＯ３０ｍｉｎ后，培养基肌酸激酶（ＣＫ）水平显著上升，细胞活力下降。预先应用ＡＴＰ、ＡＴＰ－ＭｇＣｌ２（ＡＭ）、超氧化物歧化酶（ＳＯＤ）、别嘌呤醇（ＡＬＬＯ）和还原型谷胱甘肽（ＧＳＨ）均使ＣＫ水平及细胞活力维持或接近正常水准。提示：氧自由基对人类小肠上皮细胞具有直接毒性作用，体外应用ＡＴＰ、ＡＭ、ＳＯＤ、ＡＬＬＯ及ＧＳＨ能有效地防治活性氧对小肠上皮细胞的毒性损伤。
Primary cultures of fetal human intestinal epithelial cell (IEC) were used in vitro to study the injurious effects and their prevention of the oxygen matabolites derived from xanthine/xanthine oxidase(X/XO)system.The results showed that the creatine-kinase (CK) level of the culture medium elevated significantly and the cell viability lowered in thirty minutes following adding xanthine and xanthine oxidase to the cultures. However,pretreatment with ATP. ATP-MgCl2.superoxide dismutase (SOD). allopurinol(ALLO)or reduced glutathione(GSH)could maintain or approach a normal CK level and a normal cell viability in the cultures.It is suggested that reactive oxygen metabolites have a direct toxic effect on human IEC,and the injuries can be effectively mitigated by pretreatment with ATP ATP- MgCl2, SOD,ALLO or GSH.
出处
《中华小儿外科杂志》
CSCD
1994年第5期295-297,共3页
Chinese Journal of Pediatric Surgery
基金
国家自然科学基金
关键词
肠上皮细胞
氧
自由基
抗氧剂
Intestinal epithelial cell
Oxygen radical
Antioxidants
