摘要
目的 研究体外循环血清对肺表面活性物质相关蛋白A(SP A)的影响和己酮可可碱 (PTX)对其作用。方法 实验分为 3组。将培养的肺泡Ⅱ型上皮细胞 (AT Ⅱ )分别与CPB术前 (对照组 )、术后 (损伤组 )血清共同孵育 ,PTX组除AT Ⅱ与CPB术后血清外 ,再加入 1 0 0mmol/L的PTX。检测AT Ⅱ形态改变和SP A、SP AmRNA表达的变化。结果 损伤组细胞形态改变 ,SP A、SP AmRNA较对照组明显降低 ,PTX有明显的保护作用。结论 CPB术后血清能影响AT Ⅱ中SP A翻译和转录 ,这可能是术后肺表面活性物质 (PS)质和 /或量异常的重要原因。PTX能减轻CPB后血清对SP
Objective To explore the injurious effect of the serum drawn from patient under cardiopulmonary bypass (CPB) on pulmonary surfacant associated protein A(SP A) and the preventive effect of pentoxifylline (PTX) on the protein. Methods The cultured rat alveolar epithelial cell type Ⅱ(AT Ⅱ) were incubated with CPB serum to observe the change of the cell morphology, and the expressions of SP A and SP A mRNA. Results Traumatic changes and the decrease of SP A and SP A mRNA expressions were found in AT Ⅱ cells cultured with CBP serum. PTX exerted protective effect on the cells. Conclusion The serum after CPB can directly injure rat AT Ⅱ cells in vitro , and inhibit the SP A expression at transcription and translation levels, which probably is an important reason for the quantitative and qualitative abnormality of pulmonary surfactant after operation. PTX may alleviate the inhibitory effect of CPB serum on SP A.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第3期340-342,共3页
Journal of Third Military Medical University
