摘要
运用受体的放射配基结合分析，研究了不同月龄大鼠肝低密度脂蛋白（ＬＤＬ）受体功能。结果表明，４月龄、２０月龄、２６月龄大鼠肝ＬＤＬ受体最大结合容量（Ｒｍａｘ）分别为２７．６６±８．８２、１６．７９±１０．３２，１５．１７±９．５７（ｎｇ／ｍｇ匀浆蛋白），２０月龄、２６月龄组明显低于４月龄组（Ｐ＜０．０１）；受体解离常数（ＫＤ）分别为６．６２±１．９９、８．７７±２．３６、９．５９±２．０４（μｇ／ｍｌ匀浆），１２０月龄、２６月龄明显高于４月龄（Ｐ＜０．０５及０．０１）。提示老年大鼠肝ＬＤＬ受体结合位点数减少，亲和力下降，受体功能衰退。结果还表明，２０月龄、２６月龄大鼠血清ＴＣ和ＬＤＬ－Ｃ高于４月龄组（Ｐ＜０．０１）。老年大鼠肝ＬＤＬ受体功能衰退可能是其血清ＬＤＬ升高的重要原因。
Abstract Liver receptors of low density lipoprotein (LDL) were studied with radioligand binding assay of receptors (RBA) and serum total cholesterol (TC) and LDL cholesterol (LDL-C) were measured in young (4-month) and aged (20-month, 26-month) rats. The Rmax and KD value of liver LDL receptors binding in aged group were respectively lower (P<0.01) and higher (P<0.05-0.01) compared to those in young rats. The results suggested that the number of the receptors binding decreased and affinity declined in the aged. The results also showed that serum TC and LDL-C levels were higher(P<0.01) in aged group than those in young rats. These data reflected that the changes of liver LDL receptors might be one of the main factors resulting in the rise of serum LDL in aged rats.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1994年第1期3-5,共3页
Chinese Journal of Geriatrics
