摘要
将去除大部分3’端非编码区的LDL受体cDNA片断插入pSVL质粒,获得重组质粒pLDL RN,并在LDL受体阴性的中国仓鼠卵巢细胞CHO 7a-1细胞中获得表达。表明3’端非编码区对LDL受体基因表达并非必不可少。
A plasmid pLDL RN, recombined by insertion of plasmid pSVL with LDL
receptor cDNA fragment lacking most of its 3′ non-coding sequence, was expressed by LDL
receptor negative-CHO 7a-1. It is apparent that the 3′ non-coding sequence is not absolutely
necessary for the expression of the LDL receptor gene.
关键词
低密度脂蛋白
受体
仓鼠
卵巢细胞
Low density lipoprotein receptor (LDLR)
gene expression
Chinese hamster ovary cells (CHO)
