摘要
目的 研究NF κB受体活化因子配体(RANKL)和骨保护蛋白(OPG)) mRNA在咬合创伤大鼠牙槽骨组织中的相对表达水平,探讨RANKL和OPG在咬合创伤牙槽骨改建中的意义。方法 将30只大鼠随机分为对照组和实验组组。实验组大鼠左侧上颌第1磨牙咬合面上粘结厚度为1mm的方丝,用Super bond复合树脂堆积法粘结,形成同侧下颌第1磨牙的咬合创伤模型;对照组未作粘结处理。在1、3、7、14、28天处死大鼠后,提取其左下颌第1磨牙区牙槽骨组织总RNA。用RT PCR检测RANKL和OPGmRNA表达水平。结果 持续性咬合创伤导致牙槽骨内RANKL mRNA表达增强,与对照组在第1、7天比较,差异有统计学意义(P<0 05);OPG mRNA表达减弱,与对照组在第14、28天比较差异有统计学意义(P<0 05)。实验组RANKL/OPG mRNA值偏高,但与对照组比较差异无统计学意义(P>0 05)。结论 咬合创伤后RANKL mRNA表达增强、OPG mRNA表达减弱,可能与牙周组织适应性改建有关。
Objective To investigate the expression change of receptor activator of NF κB ligand (RANKL) and osteoprotegerin (OPG) mRNA in rat alveolar bone and the possible mechanism of alveolar bone resorption under traumatic occlusion. Methods Thirty SD rats weighed 270g±20g were used and divided into 6 groups, of which one group was as control. A square wire thickened about 1mm was bonded with Super bond composite resin on to the occlusal surface of left upper first molar of the rats in experiment groups. The animals were executed after 1,3,7,14,28 days. The left jaw were processed for the study of the expression of RANKL and OPG mRNA with RT PCR. Results Expression of RANKL and OPG mRNA in alveolar bone was significantly different between experiment and control groups. During occlusal trauma, expression of RANKL and OPG mRNA enhanced in 1 day group. The ratio of RANKL/OPG mRNA expression raised in 1、14 and 28 day groups. Conclusion The ratio of RANKL/OPG mRNA expression changed during occlusion trauma. RANKL and OPG might be involved in the restructure of periodontal tissue after traumatic occlusion
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2005年第6期509-511,共3页
Medical Journal of Chinese People's Liberation Army
基金
北京市自然科学基金资助项目(编号 7053077)
