摘要
目的探讨脑桥蓝斑内神经元、星形胶质细胞和小胶质细胞是否参与咬合升高后信息的传递。方法64只雄性大鼠随机分为正常对照组(n=8)、假操作对照组(n=8)和咬合升高刺激组(n=48)。咬合升高刺激组大鼠双侧上颌第一磨牙咬合升高约0.5mm,以成活时间(1、2、4、8、24、72h)又分为6个亚组(每个亚组8只)。常规固定、取材、恒冷箱切制含脑桥蓝斑的脑桥吻侧冠状切片(片厚30μm),进行抗Fos-OX42或抗GFAP免疫荧光染色,激光共聚焦显微镜观察。结果正常对照组和假操作对照组没有差异,合并为对照组。对照组Fos表达很稀疏,实验组Fos表达明显增加,2h达到高峰,然后下降。对照组OX42和GFAP表达很微弱,不同时间点没有明显变化,但实验组OX42和GFAP在8h出现明显增强的阳性反应,24h达到高峰,72h下降。结论咬合升高激活蓝斑内神经元、星形胶质细胞和小胶质细胞,它们可能参与咬合升高后伤害性信息传入中枢的处理。
AIM ： To investigate the effect of bite - rising on locus coeruleus in loons of rat. METHODS ： 64 SD rats were divided into 1,2,4,8,24,72 hour experimental groups, one control group and one shamoperated group （n = 8 ）. The occlusal surface of the first maxillary molar of experimental rats was bilaterally raised about 0. 5wan with the square steel, sham - operated SD without binding, normal 8 SD rats without operation as control. Methods of double - labeling immunofluorescence and laser confocal microscopy were used to observe the distribution of FOS - OX42 and GFAP in rats loons. RESULTS： In locus coeruleus ,no difference was found between normal control groups and sham-operated control groups. Compared with the control group, increased expression of Fos and GFAP were found in the subnucleus locus coeruleus of the experimental rats. The peak expression time were at 2 hour and 24 hour respectively. Weak positive expression of OX42 was found in the control group. However, strong positive expression was found at 8 hour and the peak expression at 24 hour in the experimental rats. CONCLUSION： The neurons,microglias and astrocytes in parabrachial nucleus of loons were involved in bite -rising response. The response of Fos, GFAP and OX42 peaked in rats at 2 hour, 24 hour and 24 hour respectively.
出处
《牙体牙髓牙周病学杂志》
CAS
2006年第8期446-448,共3页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助课题(30572066)
