摘要
用高效凝胶渗透色谱法测定了脱乙酰化程度为90％壳聚糖样品的分子量,并与粘度法测定的分子量结果进行了比较。实验数据表明:TSK SW系列分离柱在流动相为0.1mol/L醋酸钠和醋酸介质中,可以较有效地分离壳聚糖。用葡聚糖为标准样品得到的标准曲线,需要经过普适校正后,才能比较可靠地用于测定壳聚糖样品的分子量。
The molecular weight of chitosan was determined by HPGPC on TSK SW type column. The experimental data showed that the separation efficiency of TSK SW type column is quite high for Dextran standard and chitosan samples. A good linear fit of logMw-tR and log[η]Mw-tR was obtained while dex-tran was used as standard samples. In comparison with the Mv, it was concluded that the universal calibration is necessary to the molecular weight determination of chitosan.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1994年第4期242-244,共3页
Journal of China Pharmaceutical University
