摘要
目的:探讨小鼠胎肝组织中干细胞抗原1阳性的细胞(Sca 1+细胞)向心肌细胞分化的潜能。方法:取妊娠14.5 d的小鼠胎肝,制作细胞悬液,用单克隆免疫磁珠细胞分离技术分离Sca 1+细胞,PCR鉴别Y染色体性别决定区域(Sry)基因序列;将2×103 个雄性小鼠Sca 1+细胞输注给经致死剂量60Co(10 Gy)全身照射的雌性小鼠体内,于移植后2个月,处死受体小鼠,取心肌组织固定、制片,免疫组织化学染色和荧光原位杂交检测雌性受体小鼠心肌组织内供体小鼠胎肝Sca 1+细胞向心肌细胞分化的情况。结果:在受体小鼠心肌组织内发现存在Y染色体阳性的供体来源的细胞,呈现心肌组织的部分特征,表型为Desmin+/Flt 1-/CD45-/F-4/80。结论:小鼠胎肝Sca 1+细胞(其中绝大部分是造血干细胞)具有向心肌组织细胞分化的潜能。
Objective:To explore potential differentiation from antigen-1 positive stem cells (Sca-1 +cells) in murine fetal liver tissue into cardiomyocytes in vivo. Methods: Sca-1 + cells from the murine fetal liver of 14.5 d were separated by Magnetic cell sorting (MACS).The sequence of the sex determination region of the Y chromosome was determined by PCR.Sca-1 + cells (2×10 3) of male mice were transfused to female mice receiving 8-12 weeks irradiation at a lethal dose of γ-ray(10 Gy) from a 60Co source.Two months later,the mice were killed and the hearts were removed to make slices. Fluorescence in situ hybridisation (FISH) tests with a Y chromosome probe were used to detect sex of the cells.Immunohistochemistry with antibodies of desmin,Flt-1,white blood cell common antigen CD45 and macrophage F 4/80 were used to detect tissue characteristics of cardiomyocytes. Results: Cells with Y chromosome were found in the cardiomyocytes of female mice transplanted with Sca-1 + cells,and showed phenotypic characteristics of Desmin +/Flt-1-/CD45-/F- 4/80 at the same time.Conclusion: Sca-1 + cells from the murine fetal liver are mostly hematopoietic stem cells,having the potential of differentiating into cardiomyocytes.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第4期371-375,共5页
Academic Journal of Second Military Medical University
基金
广东省科技计划基金(99M01204G)
广州市科技计划基金(2001 2 037 01).
