摘要
目的 探讨骨髓间充质干细胞 (mesenchymalstemcells,MSC)对合并局部放射损伤难愈性创面的作用及其机理研究。方法 成年健康贵州小香猪 8只 ,3%戊巴比妥钠静脉麻醉 ,电子线照射脊柱两旁皮肤 ,深度 1cm ,立即在受照射创面打孔 ,直径 2cm ,每侧 8～ 10个。一侧为对照组 ,局部注射生理盐水 ;一侧为MSC组 ,局部注射自体MSC。采用早期贴壁法分离小香猪骨髓MSC ,将自体的MSC移植到局部合并 2 0Gy的放射损伤创面 ,采用激光共聚焦、胶原定量、ELSIA、细胞划痕实验、RT PCR等方法 ,观察了MSC对合并放射损伤创面的促愈作用及其促愈的机理。结果 将自体的MSC移植到局部合并 2 0Gy创面后能加快创面愈合的速度 ,使愈合的时间比对照组提前了 3～ 4d ,肉芽组织形成丰富 ,胶原合成增加。其促愈的机理与MSC分泌IL 8、IL 6、G CSF等细胞因子吸引和趋化炎症细胞和修复细胞向创面迁移 ,启动修复 ,增加伤口中修复细胞 (成纤维细胞 ,血管内皮细胞 )的数量有关 ,同时MSC在创伤局部微环境的作用下 ,基因和蛋白表达发生变化 ,尤其是I型胶原基因表达和蛋白合成增加 ,提示在局部微环境作用下 ,MSC可分泌细胞外基质或 /和直接分化为成纤维细胞参与组织的修复。结论 MSC对局部合并放射损伤的创面有明显有效的促愈效应 。
Objective To explore the effects of swine bone marrow derived mesenchymal stem cells(MSC) on the repair of skin wound combined local irradiation injury and theirs mechanism. Methods MSC were isolated from the adult swine bone marrow and autologous MSC were directly transplanted into the local site full thickness skin incision round wounds combined with 20 Gy irradiation.By means of laser confocal microscopy, quantitive collagen mearsurement, ELSIA, fibroblasts scrap test, RT-PCR technology and so on in order to observe the effects of MSC promote the repair of wound and theirs mechanism. Results Transplanted autologous MSC into local skin wound combined with 20 Gy irradiation could accelerate the speed of wound healing, the healing time moved up 3-4 days. The granule tissues were abundant in the wounds and the content of collagen was increased in MSC groups. On one hand, MSC attracted the inflammatory cells and repaired cells to immigrate into wounds by theirself or secretion of IL-6, IL-8 and G-CSF and thus initated the repairing processes. On the other hand, the wound microenviroment induced the proliferation and changes of I collagen mRNA expression of MSC showed that MSC could secrete extracellular matrix or differentiate into fibroblast. Conclusion Local transplanted MSC promte the repair of skin wounds combined with irradiation injury. The promotion effects were achieved by the interration betweem MSC and wound microenviroment.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2002年第23期1632-1636,共5页
National Medical Journal of China
基金
国家重点基础研究发展规划基金资助项目(G19990 5 42 0 5 )
