摘要
为了解胎肝干细胞是否具有向神经组织细胞分化的潜能 ,本研究采用免疫磁珠法分离雄性胎鼠肝组织中干细胞抗原 1(stemcellantigen ,Sca 1)阳性细胞 ,尾静脉注射Sca 1+ 细胞 (2 0× 10 3个 小鼠 )到经致死剂量 (10 0Gy)放射线照射的 10～ 12周C5 7BL 6J雌性小鼠。免疫组化和FISH双染色检测结果显示移植 2、4、6月后受体雌鼠脑组织内存在大量Y染色体阳性细胞 ,约占脑组织总细胞为 4 5 %± 0 5 % ,分布包括 :侧脑室脉络膜丛、脑室室管膜、大脑白质灰质、小脑等。这些Y染色体阳性细胞表达神经组织细胞特异标志 ,如神经元特异核蛋白 (Neuron specificnucleiprotein ,Ne uN)、神经纤维细丝蛋白M(neurofilament 16 0Ka,NF M)、微管蛋白Ⅲ (tubulinⅢ ,TuJ 1)、或者胶质纤维酸性蛋白 (Glialfibrillaryacidicprotein,GFAP)等 ,其中Y染色体和NeuN均阳性的细胞为 1 2 %± 0 3%、Y染色体和GFAP均阳性的细胞为 1 0 %± 0 2 %。这说明胎肝Sca 1+ 细胞能迁移进入脑组织 ,并且分化成神经细胞和星形胶质细胞。
To study whether Sca-1 + cells from fetal liver can differentiate into neural cells, Sca-1 + cells from male fetal liver isolated with a magnetic cell sorting kit were transplanted into lethally irradiated female mice (2×10 3 cells/mouse). FISH and immunohistochemistry double-staining analysis showed that there existed many male cells in brains of female recipients 2, 4, 6 months after transplantation, including white and gray matters, forbrain, midbrain, the ependyma of the ventricular system, and the choroid plexus of the lateral ventricle. Ratio of Y chromosome-positive cells in brain is (4.5～0.5)%. These cells expressed neural-specific antigens such as NeuN (Neuron-specific nuclei protein), TuJ-1(Tubulin beta Ⅲ), NF-M(Neurofilament M) and GFAP(Glial fibrillary acidic protein). Ratio of both Y chromosome and NeuN positive cells (1.2±0.3)%, ratio of both Y chromosome and GFAP positive cells (1.0±0.2)%. These results indicate that murine fetal liver Sca-1 + cells can differentiate into neural cells in brains of adult mice.
出处
《基础医学与临床》
CSCD
北大核心
2004年第3期299-302,i002,共5页
Basic and Clinical Medicine
