期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

线粒体DNA5178A/C多态性与2型糖尿病人血脂水平的关系 被引量：1

Association of the Mitochondrial DNA 5178A/C Polymorphism with Serum Lipid Levels in Patients with Type 2 Diabetes Mellitus
暂未订购
导出
摘要 目的研究线粒体DNA5178A/C多态性在2型糖尿病人与正常人中的分布情况,以及线粒体DNA5178A/C多态性与2型糖尿病人血脂水平的相关性。方法采用聚合酶链反应(PCR)和限制性片段长度多态性(RELP)分析法对2型糖尿组(174例)及正常对照组(207例)进行基因分析,同时测定2型糖尿病组患者的血脂水平。结果线粒体DNA5178A基因型在2型糖尿病组及正常对照组中的比例分别为35%(61/174)、32%(66/207),5178C基因型的比例分别为65%(113/174)、68%(141/207);2型糖尿病组中5178A基因型与5178C基因型血清HDLC、LDLC、TG、apoA、apoB、Lp(a)水平无显著性差异,5178A基因型血清胆固醇水平低于5178C基因型,两型之间有显著性差异(P<0.05)。结论5178A/C多态性与2型糖尿病的发病未见相关性,但5178A基因型在2型糖尿病人中可能有抗动脉粥样硬化作用。 Objectives To study the prevalence of mitochondrial DNA 5178A/C polymorphism in type 2 Diabetes mellitus patients and healthy controls among Chinese Han population of HuBei Province and its association with serum lipid levels in type 2 diabetics. Methods PCR and RELP techniques were used to examine 174 patients with type 2 diabetes(diabetic group) and 207 healthy people (control group) to analyse the mitochondrial gene and detect the serum lipid levels in patients with type 2 diabetes mellitus.Results The frequency of 5178A in diabetic group and control group were 35% and 32% respectively and in the 5178C were 65% and 38% respectively. There were no significance difference between the two groups, for the frequencies of 5178A/C polymorphism genotypes The serum levels of LDL C、HDL C、TG、apoA、apoB、Lp(a) had no significance difference in the 5178A and 5178C but the serum TC level was significantly lower in the 5178A than 5178C.Conclusion the MtDNA 5178A/C polymorphism may not be related to the etiology of type 2 diabetes. However,5178A seems to have an antiatherogenic effect, at least in type 2 diabetic.
作者 古今刚 周新 李霞 李东 刘松梅 胡飘萍
机构地区 武汉大学中南医院临床基因诊断中心
出处 《医学新知》 CAS 2005年第2期40-41,44,共3页 New Medicine
基金 武汉市攻关课题(20016009107) 湖北省自然科学基金资助项目(2004ABA163)
关键词 血脂水平 糖尿病人 A/C 线粒体 聚合酶链反应(PCR) 限制性片段长度多态性 抗动脉粥样硬化作用 2型糖尿病 正常对照组 显著性差异 C基因型 HDL-C LDL-C Lp(a) 胆固醇水平 分布情况 基因分析 同时测定 APOA apoB 相关性 Mitochondria Polymorphism Type 2 diabetes mellitus
分类号 R587.1 [医药卫生—内分泌]
  • 相关文献

参考文献6

  • 1Tanaka M, Gong J S, Zhang J, et al. Mitochondrial genotype associated with longevity. Lancet, 1998, 351(9097) :185.
  • 2Matsunaga H, Tanaka Y, Tanaka M, et al. Antiatherogenic mitochondrial genotype in patients with type 2 diabetes. Diabetes Care, 2001, 24(3) :500.
  • 3Katsumasa Takagi ,Yoshiji Yamada, Jian-Sheng Gong, et al. Association of a 5178C→A (Leu237Met) polymorphism in the mitochondrial DNA with a low preva lence of myocardial infarction in Japanese individuals. Atherosclerosis,2004, 175(1) : 281.
  • 4Van den Ouweland J M , Lemkes H H, Ruitenbeek W, et al .mutation in mitochondrial tRNAleu(uuR) gene in a large pedegree maternally transmitted typeⅡ diabetes mellitua and deafness. Nature genetics ,1992 ,1(5) :368.
  • 5Clinnery P F, Howell N, Andrews R M ,et al .Clinical mitochondrial genetics. J Med Genet,1999,36(6) :425.
  • 6Yao Y G, Kong Q P, Zhang Y P. Mitochondrial DNA 5178A polymorphism and longevity.Hum Genet ,2002, 111(4～5):462.

同被引文献12

  • 1Tanaka M,Gong JS,Zhang J, et al. Mitochondrial genotype associated with longevity [J].Lancet, 1998, 351 (9097) :185.
  • 2Matsunaga H, Tanaka Y, Tanaka M, et al. Antiatherogenic mitochondrial genotype in patients with type 2 diabetes [J]. Diabetes Care,2001,24(3) :500-503.
  • 3Takagik G, Yamada Y, Gong JS, et al. Association of a 5178C→A (Leu237Met) polymorphism in the mitoch(sndrial DNA with a low prevalence of myocardial infarction in Japanese individuals [J]. Atherosclerosis, 2004, 175 (1) :281- 286.
  • 4Mark J, Rieder, Scott L, et al. Automating the identification of DNA variations using quality-based fluorescence re-sequencing: analysis of the human mitochondrial genome[J]. Nucleic Acids Research, 1998,26 (4) : 967 973.
  • 5Levine RL, Mosoni L, Berlett BS, et al. Methionine resi dues as endogenous antioxidants in proteins[J]. Proc Natl Aead Sci USA,1996,93(150) :36-40.
  • 6Wang D,Taniyama M,Suzuki Y, et al. Association of the mitochondrial DNA 5178 A/C polymorphism with maternal inheritance and onset of type 2 diabetes in Japanese patients[J]. Diabetes, 2001,109 : 361-364.
  • 7Kokaze A, Ishikawa M, Matsunaga N, et al. Longevity associated mitochondrial DNA 5178 A/C polymorphism a1 d blood pressure in the Japanese population[J]. Hum Hypertens, 2004,18 : 41-45.
  • 8Kokaze A, Ishikawa M, Matsunaga N, et al. Association of the mitochondrial DNA 5178 A/C polymorphism with strum lipid levels in the Japanese population[J]. Hum Genet,2001,109:521-525.
  • 9Kokaze A,Ishikawa M,Matsunaga N,et al. Longevity associated mitochondrial DNA 5178 A/C polymorphisn modulates effects of daily drinking and cigarette consumption on serum triglyceride levels in middle-aged Jananese men[J]. Exp Gerontol, 2003,38 : 1071-1076.
  • 10Kokaze A, Yoshida M, Ishikawa M, et al. Longevity-associated mitochondrial DNA 5178 A/C polymorphism is a sociated with intraocular pressure in Japanese men[J]. Clin Exp Ophthalmol,2004,32 : 131-136.

引证文献1

二级引证文献1

医学新知
2005年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部