自愿运动对快速老化小鼠学习记忆能力和海马生长相关蛋白43的影响被引量：7

Effects of voluntary exercise on learning ability,memory and hippocampus growth-associated protein 43 expression in senescence-accelerated prone mouse

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摘要目的观察自愿运动对快速老化小鼠(senescence-accelerated mouse prone 8,SAMP8)学习记忆能力和海马生长相关蛋白43(growth-associated protein-43,GAP43)表达的影响,探讨运动提高认知能力延缓衰老的机制。方法 60只3个月龄雌性SAMP8小鼠随机平均分为跑笼环境组(RCE组)和标准环境组(SE组)。饲养3个月后,用Morris水迷宫测试小鼠的寻找平台潜伏期及搜索策略。行为学测试后,各组分别取10只小鼠的鼠脑用RT-PCR法检测海马GAP43mRNA的表达;取10只小鼠的鼠脑用免疫印迹实验检测海马GAP43蛋白的表达;剩余10只取鼠脑用免疫组织化学实验观察海马GAP43免疫反应产物的表达。结果 Morris水迷宫实验表明RCE组小鼠寻找平台潜伏期较SE组缩短(P<0.01,P<0.05),在第Ⅰ象限游泳时间比SE组延长(P<0.01),在第Ⅳ象限游泳时间比SE组缩短(P<0.05)。RCE组与SE组比较,海马GAP43表达增加(P<0.01)。结论自愿运动能提高SAMP8小鼠学习记忆能力,其机制可能与运动能促进海马GAP43表达有关。 Objective To observe the effects of voluntary exercise on the learning ability, memory and hippocampus growth-associated protein 43 （GAP43） expression in senescence accelerated prone mouse （SAMP8）, so as to explore the possible mechanism of exercises on improving the cognitive ability and delaying aging. Methods A total of 60 three-month old female SAMP8 mice were evenly assigned to running cage environment （RCE） group and standard environment （SE） group at random. After three months, Morris water maze test was used to test the platform-seeking latency and search strategy. Then 10 mice were sacrificed in each group for RT-PCR analysis of hippocampus GAP43 mRNA expression, 10 for Western blotting analysis of hippocampus GAP43 protein expression, and 10 for immunohistochemistry staining of hippocampus （;AP43 expression. Results Morris water maze test showed that RCE mice had a significant shorter platform-seeking latency than SE mice（P〈0.01, P〈0.05）, and RCE mice had a significant longer time in the first quadrant （P〈0.01） and a shorter time in the fourth quadrant （P〈0.05） compared with SE mice. RCE mice had a significantly higher GAP43 expression in the hippocampus compared with SE mice （P〈0.01）. Conclusion Voluntary exercise can improve the learning ability and memory of SAMP8, which might he associated with the increase of GAP43 in the hippocampus.

作者苑振云姜向明王铭维顾平杨秀芬苏冠丽杨涛李斌

机构地区河北医科大学第一医院

出处《第二军医大学学报》 CAS CSCD 北大核心 2012年第1期48-52,共5页 Academic Journal of Second Military Medical University

基金河北省卫生厅课题(20090338) 河北省引进留学人员经费资助项目~~

关键词运动快速老化小鼠环境 GAP-43蛋白 exercise senescence accelerated mouse environment GAP-43 protein

分类号 R749.16 [医药卫生—神经病学与精神病学]

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