摘要
目的:脑突触后致密区蛋白95具有调节人脑认知活动中突触功能和结构的作用,观察复方金思维干预实验动物致密区蛋白95表达以及学习记忆能力的变化,并与安慰剂及盐酸多奈哌齐干预相对照。方法:实验于2004-11-15/2005-02在北京中医药大学东直门医院药理实验室及首都医科大学宣武医院神经生化室进行。取雄性SD大鼠32只,随机分为4组,每组8只:生理盐水组、模型组、盐酸多奈哌齐组和金思维组。生理盐水组大鼠海马注入2μL生理盐水,其他3组大鼠缓慢注入2μL淀粉样β蛋白1～42(5g/L),复制阿尔茨海默病模型。造模后3d开始给药,盐酸多奈哌齐组按0.92mg/kg的剂量灌胃;复方金思维(由中药人参、肉苁蓉、石菖蒲及郁金等组成)配置成0.36g/mL的浓度,按0.7μL/g的剂量给药;模型组及生理盐水组给3mL的双蒸水,均1次/d,共4周。4周后,以Morris水迷宫测试大鼠学习期间的逃避潜伏期和游泳距离以及记忆测定中的记忆能力,评价行为学变化;以免疫组化检测大鼠海马CA1区、皮质突触后致密区蛋白95表达的变化。结果:经补充后32只大鼠进入结果分析。①Morris水迷宫的学习、记忆测试结果:模型组大鼠逃避潜伏期、游泳距离及记忆能力显著低于生理盐水组(P<0.01),盐酸多奈哌齐组、金思维组大鼠逃避潜伏期、游泳距离及记忆能力较模型组高(P<0.
AIM: Protein 95 in post synapse dense area of brain tissue can regulate the function and structure of synapse in cognitive activity of human brain.To observe the effect of fufang jinsiwei on the expression of protein 95 in post synapse dense area and learning and memory ability compared with placebo and Aricept.MEHTODS:The experiment was performent in Pharmacological Laboratory of Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine and Neurobiochemical Laboratory of Xuanwu Hospital of Capital University of Medicial Sciences from November 2004 to March 2005. Totally 32 male SD rats were divided randomly into saline group, model group, Aricept group and jinsiwei group(n=8).Rats in saline group were injected with 2 μ L model saline, which rats in other three groups were injected with 2 μ L(5 g/L)β amyloid1 42 injection into hippocampus to induce Alzheimer disease models. Three days after surgery, intragastric administration of jinsiwei (composed with ginseng, desertliving cistanche, shichangpu and zedoary turmeric root tuber, etc.) at a dosage of 0.7 μ L/g (0.36 g/mL) and Aricept at a dosage of 0.92 mg/kg was taken in jinsiwei group and Aricept group, respectively; saline group and model group were treated with 3 mL distilled water once per day for 4 weeks. Four weeks later, Morris water maze test was used to evaluate the latent period for escaping learning and swimming distance, and memory ability and praxiological changes; Immunohistochemistry staining was used to detect the expression of protein 95 in post synapse dense area of cortex and hippocampus CA1 region.RESULTS: A total of 32 rats were involved in the result analysis after supplement.① Morris water maze test showed the escaping latency, distance and memory ability in model group were decreased significantly than those in saline group(P< 0.01), and jinsiwei group and Aricept group(P< 0.05); there was no significant difference between jinsiwei group and Aricept group(P >0.05).② The expression of protein 95 in post synapse dense area of cortex and hippocampus CA1 region was decreased in model group as compared with that in saline group(P< 0.01), jinsiwei group and Aricept group(P< 0.05); there was no significant difference between jinsiwei group and Aricept group(P >0.05).CONCLUSION:The decreasing expression of protein 95 in post synapse dense area indicates the function of brain synapse is attenuated and injured; the increasing expression of protein 95 in post synapse dense area indicates the function and compliance of brain synapse is recovery.The result shows fufang jinsiwei can improve the function and structure of synapse.
出处
《中国临床康复》
CSCD
北大核心
2005年第17期139-141,共3页
Chinese Journal of Clinical Rehabilitation
基金
教育部高等学校博士学科专项科研基金(20030026011)
教育部重大科学技术研究项目(10408)~~
