摘要
1目的 研究脑缺血再灌注后生长相关蛋白 - 43(GAP- 43)和突触素 p38表达的变化规律 ,探讨中枢神经损伤后修复的可塑性。 2方法 成年健康雌性 Wistar大鼠 36只 ,随机分为实验组和对照组。采用线栓法建立大脑中动脉缺血再灌注大鼠模型 ,应用免疫组织化学方法观察脑缺血再灌注后 GAP- 43和突触素 p38的表达。 3结果 脑缺血再灌注 6 h后 ,GAP- 43表达逐渐增高 ,第 7天达高峰 ,以后逐渐降低 ,第 2 1天仍有表达。脑缺血再灌注 1d后突触素 p38表达增高 ,第 7天达高峰 ,之后逐渐降低 ,第 2 1天降至对照组水平。4结论 中枢神经系统损伤后 ,神经元具有再生和修复的可塑性 ,GAP- 43和突触素 p38是神经损伤和修复的分子标志物。
Objective To study the effect of reperfusion on expressions of growth associated protein 43 (GAP 43) and synaptophysin (p38) after middle cerebral artery occlusion in rats and the neuroplasticity of central nervous system after injury. Methods \ 36 adult female rats were divided randomly into experimental group and control group. The rats received left middle cerebral artery occlusion for 2 hours. The sections of the brains were subjected to 6 hours and 1,3,7,14,21days of reperfusion and processed by immunochemistry with antibodies against GAP 43 and p38.\ Results\ GAP 43 immunoreactivity increased at 6 hours after reperfusion, and reached the peak on the 7th day and declined in the later days, but it still remained high level on 21th day. P38 immunoreactivity increased on 1st day, reached its peak on 7th day ,and returned almost to the control level on 21st day. Conclusion \ This study suggests that there might be neuroplasticity of regeneration and repairment in central nervous system after injury. GAP 43 and p38 were molecular markers of nervous injury and regeneration. [
出处
《青岛大学医学院学报》
CAS
2000年第4期247-249,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
青岛市科委自然科学基金!资助项目 ( No.98-17)
关键词
生长相关蛋白
突触蛋白类
脑缺血
大鼠
growth associated proteins, neuronal
synapsins
cerebral ischemia
rats
