摘要
目的探讨在不同缺氧时相人视网膜色素上皮(RPE)细胞转录和表达缺氧诱导因子-1α(HIF-1α)的情况.方法原代培养人RPE细胞,经鉴定后,选择第3～6代RPE细胞进行缺氧分析,分别在缺氧6、8、16、24、48h不同时相,利用半定量RT-PCR法及免疫荧光测定法检测人RPE细胞转录和表达HIF-1α的情况.结果半定量RT-PCR法和免疫荧光法检测结果均显示缺氧可以明显诱导人RPE细胞转录和表达HIF-1α.在缺氧8h时,HIF-1α转录和表达量达高峰,并可持续至24h后;缺氧48h时,人RPE细胞代谢产物增加,细胞形态发生改变,HIF-1α转录和表达量开始下降.结论缺氧可以明显诱导人RPE细胞转录和表达HIF-1α,这可能是发生脉络膜新生血管的重要始动因素.(中华眼科杂志,2005,41:312-316)
Objective To investigate the expression of HIF-1α in human retinal pigment epithelium (hRPE) induced by hypoxia at different time points and to study the mechanism of choroidal neovascularization. Methods hRPE were isolated, cultured and identified. The changes of level of mRNA and protein of HIF-1α in hRPE at different time points after exposed to hypoxia were measured by semi-quantitative RT-PCR and immunofluorescence techniques. Results A significant increase of HIF-1α level in cultured hRPE was induced by hypoxia. After 8 hours exposed to hypoxia, the level of HIF-1α in the hRPE reached a peak and sustained for more than 16 hours. After a prolonged exposure to hypoxia, the morphology of hRPE began to change and the HIF-1α level was decreased. Conclusions The level of HIF-1α in hRPE rises obviously after exposed to hypoxia. This may play a role in the occurrence of choroidal neovascularization.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2005年第4期312-316,共5页
Chinese Journal of Ophthalmology
基金
吉林大学第二医院青年基金资助(2003)
