摘要
过氧化物酶同工酶（ＰＯＤ）是真菌遗传分析中的一个常用生化指标，在其电泳分析中染色操作是一个重要技术环节。本研究以香菇、美味侧耳为材料，比较了不同ＰＯＤ染色方法的染色效果。ＮＨ＿３Ｃｌ－联苯胺法效果最佳，醋酸一联苯胺法次之，ＡＣ－联苯胺及愈创木酚－联苯胺法效果较差。对影响染色效果的原因进行了分析。
Peroxidase isozymes (POD) are one of the frequently-used biochemical markers in genetical analysis of fungi,The staining is an important procedure in studying POD.Theefficiency was compared;among different stain recipes for POD of two edible fungi Lentinus edodes and Pleurctus sapidus. The recipe NH_3Cl-Benzidine showed the best effect,and followed the HAc-Benzidine,but the Vc-Benzidine and Guaiacol-Benzidine did not gave satisfactory results,The possible reasons for differences of stain effect were discussed.
出处
《中国食用菌》
1994年第3期15-18,共4页
Edible Fungi of China
