摘要
利用绿色荧光蛋白基因(gfp)标记示踪,研究了葡萄根癌病生防菌葡萄土壤杆菌E26菌株应用到田间后在玫瑰香葡萄(Vitisviniferacv. MuscatHumburg)根表面和根际土壤中的群体数量变化,比较了E26菌株与葡萄根癌病原菌K308菌株室内人工接种后在玫瑰香葡萄苗茎和根外植体伤口部位的附着情况。在田间自然状况下,E26菌株可以在葡萄根表面和根际土壤中存活定殖。接种 5个月后,E26在根表面的平均数量为 104cfu/g根 (鲜重),在根际土壤中的平均数量为 104cfu/g土壤(干重)。在室内,E26菌株和K308菌株分别单独接种时均能以相似水平附着在葡萄茎和根的伤口;E26和K308以相同数量同时接种时,附着在葡萄伤口细胞的K308的数量显著低于K308单独接种时所附着在葡萄伤口细胞的数量。扫描电镜显微观察证实E26菌株能够和病菌K308菌株一样附着于葡萄根部伤口处。
The dynamics of Agrobacterium vitis strain E26,a potential grapevine crown gall biocontrol agent,in Vitis vinifera cv. Muscat Humburg rhizoplane and rhizosphere under field conditionswere studiedby using gfp -marked bacterial strain to evaluate its colonization on diseasesuppression. Attachment of strain E26 and pathogen A. vitis strain K308 to plant wound cells were also compared in vitro . Colonization and survival of strain E26 in the rhizospherewere observed in field. Average population of 10 4 cfu/g of E26 was detected in fresh root and dry soil respectively 5 months after planting. Attachment of E26 and K308 was at a similar level to both stem explants and roots of Muscat Humburg in vitro . However,K308 was blocked attach to grape cells by E26 in both the stem explants and seedling root systems. Scanning electron microscope also revealed that strain E26 could attach to the wounded cells of grape root with the same manner as that of strain K308.
出处
《植物病理学报》
CAS
CSCD
北大核心
2005年第1期78-83,共6页
Acta Phytopathologica Sinica
基金
国家"八六三"高技术项目(2001AA249061)
关键词
葡萄
根癌病
生物防治
定殖
grapevine
crown gall
biocontrol
colonization
