摘要
变形斑沙雷氏菌(Serratia proteamaculans)336x是一株对小麦全蚀病具有生防作用的小麦内生细菌。为研究336x在小麦内部的定殖及分布,将336x基因组酶切片段与携带绿色荧光蛋白基因(gfp)的质粒pAD123进行连接,筛选产生荧光的质粒并转化336x菌株,获得336x的荧光标记菌株336x-gfpa。对比336x-gfpa与野生型菌株在小麦中的定殖能力及对小麦全蚀病的生防能力,发现二者无显著差异;荧光显微镜观察结果表明,336x-gfpa主要定殖于小麦根部细胞间隙和维管组织。
Serratia protearnaculans strain 336x isolated from wheat roots is an endophytic bacte- rium and has biological control activity against take-all of wheat induced by Gaeumannomyces graminis var. tritici. To elucidate colonization pattern of 336x in wheat,the genomic DNA frag- ments of 336x were inserted into pAD123 to construct pAD123-336x,in which the gfp gene was expressed under the control of 336x gene promoter. The plasmid pAD123-336x was transferred in- to 336x wild type strain by electroporation, and one mutant designated as 336x-gfpa was acquired. Colonization study and biocontrol assay revealed that 336x-gfpa had comparatively equal biocon- trol activity and colonization ability to 336x. Observation under fluorescent microscope revealed that 336x-gfpa was mainly colonized in intercellular space and vascular tissue of wheat root.
出处
《河南农业科学》
CSCD
北大核心
2013年第9期69-72,82,共5页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(31200069)
关键词
内生细菌
变形斑沙雷氏菌
绿色荧光蛋白
基因转化
定殖
endophytic bacteria, Serratia proteamaculans, green fluorescent protein（GFP）, genetransformation, colonization
