摘要
以差异离心和蔗糖密度梯度离心法提纯了在鸡胚尿囊腔中繁殖的腮腺炎病毒粒子。并用DS-PAGE分析病毒粒子的结构多肽,发现其结构多肽为11种,分子量在35K到72K之间。同时还检测到HN蛋白的多聚体和F蛋白的大亚基F1。将腮腺炎病毒分别感染Hela、Vero和CE细胞,比较这三种细胞对ME株腮腺炎病毒的敏感性,发现CE细胞是ME株的敏感宿主。用[^(35)S]蛋氨酸标记病毒感染的CE细胞,以SDS-PAGE及放射自显影法检测到腮腺炎病毒在宿主细胞中合成了至少8种多肽,分子量在26.5K到94K之间。对这些多肽在细胞中不同时期合成情况进行了研究。还用脉冲追踪(Pulsechase)技术在感染细胞中发现了F0到F这一转译后加工(Posttranslational procession)现象。此外也研究了放线菌素D和高浓度氯化钠对细胞蛋白质合成的抑制作用。
Strain ME of mumps grown in chick amniotic cavity and purified by differential centrifugation combined with sucrose gradient centrifugation. The structural polypeptides of purified virion were analyzed by SDS polyarylamide gel electrophoresis. Eleven polypeptides were found. Their molecular weight were between 35K—72K dalton. In addition polymers of HN protein and F1 the large subunit of F protein were detected. Hela, Vero and CE cells were infected with ME strain of mumps virus. The CE cell reveals the most sensitive host cell. CE cell infected with mumps virus and labeled with [^(35)S]-Met, SDS-PAGE and examined by autoradiography. It was found at least eight polypeptides were synthesis in host cells and their molecular weight were between 26.5K to 94K dalton. The time course of the synthesis of polypeptides in the cells were studies. Palse-Chase experiments demonstrated the posttranslational procession of Fo→F.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第4期244-251,共8页
Acta Microbiologica Sinica
关键词
腮腺炎病毒
多肽生物合成
Mumps Virus
Bio-synthesis of polypeptides
