摘要
以月季品种萨蔓莎为试材研究不同外植体、叶片位置、暗培养时间和激素组合对月季不定芽诱导和植株再生的影响。结果表明,月季萨蔓莎在含1.5 mg·L-1TDZ 和0.05 mg·L-1NAA 的MS 培养基上黑暗培养8 d 后转入含0.5 mg·L-1 BA 和0.01 mg·L-1NAA 的MS 培养基光下培养,再生效果最好,小叶外植体和复叶柄的再生率分别为51.8%和10%。小叶的叶位对植株再生无显著影响,暗培养时间过长,外植体易形成愈伤组织而不利于不定芽的分化,植物生长调节剂中生长素浓度升高易导致愈伤组织形成而影响植株再生。本研究所建立的不定芽直接再生系统可用于该品种的转基因遗传改良研究。
Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on adventitious bud induction and plant regeneration of Rosa hybrida cv. Samantha was investigated. The results showed that after 8 days of induction on MS medium with 1.5mg·L-1 TDZ and 0.05mg·L-1 NAA in the dark and a subculture on MS medium with 0.5mg·L-1 BA and 0.01mg·L-1 NAA under light, the best plant regeneration was obtained and the regeneration frequencies of leaflets and petioles were 51.8% and 10%, respectively. There was no significant difference in regeneration ability between leaflets at different sites of the leaves. Longer time of induction in the dark caused callus formation and the regeneration frequency was significantly reduced. High concentration of auxin resulted in callus growth which was disadvantageous to shoot regeneration.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第4期784-788,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30170666)
