摘要
金花茶（Ｃａｍｅｌｌｉａｐｅｔｅｌｏｔｉｉ）的小孢子单核期花药，经培养和继代培养６个月后的愈伤组织中，发现有少量体细胞进行减数分裂。在成效第一和第二次分裂中，同源染色体的配对和分离基本正常，最后形成四分体。该愈伤组织经石蜡切片和压片观察，发现其主要由大量的液化细胞和贮藏细胞所组成，此外，还有少部分分生细胞。没有发现进一步的分化。其染色体数目，２ｎ＝３０者占７１．７％，其余则为非整倍体。
lt was found that a few cells progress meiosis in an anther callus culture of Camellia petelotii(Merr.) Sealy cultured and subcultured for 6 months. The pairing and segregating of homologous chromosomes at the frist and second division of meiosis were conformed with PMC meiosis, and finally,the tetrad were formation. Basec on the analysis of chromosomal mornhologyand behavior,it was considered as a special somatic meiosis. The speciality and significance of this somatic meiosis were analyzed and discusscd. Observation on preparations made with paraffin sectioning and squashing showed that callus clumps composed of some meristematic cell groups,a large quantity of vaculated cells and storge cells, without further differentiation. About 71.1 percent of cells in this callus clumps showed the chromosome number 2n= 30, and the rest cells were aneuploid.
出处
《云南植物研究》
CAS
CSCD
1994年第3期263-267,共5页
Acta Botanica Yunnanica
关键词
金花菜
花药
愈细伤组织
体细胞
Camellia petelotii, Anther callus,Somatic meiosis
