摘要
用RAPD技术对礁膜、袋礁膜和宽礁膜进行了DNA多样性分析,这3种礁膜的基因组DNA分子量均约23Kb。在使用的88个随机引物中,有43个引物在所有样品中都能重复性较好地扩增出条带清晰的多态性片段,多态引物比例为48.86%,袋礁膜、宽礁膜和礁膜多态位点比例分别为55.93%、65.28%和53.14%。礁膜与宽礁膜、宽礁膜与袋礁膜以及礁膜与袋礁膜种间遗传距离分别为0.4118,0.4148,0.4375。通过对遗传距离分析,结果表明采自3个不同海区的样本为同一礁膜属的3个种,与传统分类鉴定结果相吻合。UPGMA和NJ法构建的种间分子系统树一致,结果是宽礁膜和礁膜之间的亲缘关系最近,宽礁膜与袋礁膜之间的亲缘关系较远,礁膜与袋礁膜之间的亲缘关系最远,这与宽礁膜与礁膜共有形态特征较多,袋礁膜与宽礁膜、礁膜共有形态特征少相验证。
Random amplified polymorphic DNA markers were used to analyse the DNA diversity of Monostroma nitidum, M. latissimum and M. angicava. The size of genomic DNA of each species was about 23 kb. 43 primers screened from 88 random primers generated repeatedly clear polyotripic bands in all samples, and the proportion was 48.86%. The polymorphic sites proportion of M. nitidum, M. latissimum and M. angicava were 53.14%, (65.28%) and 55.93% respectively. The inter-species genetic distances among those species (M. nitidum, M. latissimum and M. angicava) were 0.411 8, 0.414 8 and 0.437 5 respectively. The result showed that these samples coming from three different marine area were the same genus but different species. Those were consistent with the conventional systemic identification. The molecular phylogenetic tree constructed by UPGMA method coincided with that constructed by NJ method. The result also indicated that the genetic relationship between M. latissimum and M. nitidum was the nearest, that between M. latissimum and M. angicava was farther, and that between M. nitidum and M. angicava was the farthest. The result also testified the fact that M. nitidum and M. latissimum shared more common characteristics in morphology, while M. angicava shared less common characteristics in morphology with M. nitidum and M. latissimum.
出处
《上海水产大学学报》
CSCD
北大核心
2005年第1期6-11,共6页
Journal of Shanghai Fisheries University
基金
浙江省科学技术厅科技兴海专项基金(2002C23014)
