摘要
切割后离体芽苗极易受病菌侵染而发生腐烂变质,严重影响着当前芽菜采后运输和销售。为此,本试验以豌豆芽苗为试材,研究了机械伤害和外施不同浓度(±)茉莉酸(JA)处理对豌豆幼苗膜脂过氧化的影响。结果表明,伤害和1～100ìmol·L-1JA处理可以显著提高苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性,同时使活性氧代谢加强,O2·产生速率和H2O2含量迅速增加,相应的抗氧化酶(SOD、CAT、APX、POD)活性提高。-适宜浓度JA(1～10ìmol·L-1)处理可有效诱导防御反应产生,同时,通过提高抗氧化酶活性使膜脂过氧化程度减轻;高浓度JA(100ìmol·L-1)处理造成植物活性氧代谢失调,膜脂过氧化加剧。综合分析认为,10ìmol·L-1JA是诱导豌豆幼苗防御反应产生的适宜处理浓度。
The effects of wounding and exogenous-applied jasmonic acid (JA) on the membrane lipid peroxidation were investigated in pea seedlings (Pisum sativum L.). The results showed that the activities of both phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) significantly increased by wounding and application of JA. The metabolism of reaction oxidative species (ROS) was enhanced, especially O2·and H2O2 increased rapidly. The antioxidant enzymes such as supiroxide dismutase - (SOD), catalase (CAT), ascrobate peroxidase (APX) and peroxidase (POD) were also activated. Pretreatment of JA of 1-10 ìmol·L-1 could effectively induce plant defense response, and thus decrease the level of cell membrane lipid peroxidation. However, high concentration of JA (100 ìmol·L-1) resulted in unbalance of metabolism of ROS and promote lipid peroxidation. It is concluded that JA, under the suitable concentration, can induce pea seedlings defense response to wounding.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第2期388-393,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30270918)
国家"863"计划资助项目(2003AA241170)
