摘要
目的 :探讨卵巢癌多药耐药 (multidrugresistance ,MDR)细胞的免疫逃逸机制。方法 :利用三苯氧胺 (TAM)和维拉帕米 (VRP)为逆转剂 ,运用MTT法分析了TAM、ARP的逆转效应。采用流式细胞仪技术检测耐药亚株COC1/DDPMDR逆转前后及亲本株COC1细胞的HLA -Ⅰ、HLA -Ⅱ、B7-1、B7-2的表达。结果 :COC1/DDP和COC1均表达较强的HLA -Ⅰ类抗原 ,而HLA -Ⅱ、B7-1、B7-2的表达较较低。但COC1/DDP的B7-1、B7-2抗原的表达却高于COC1。经TAM、VRP作用后 ,COC1/DDP的耐药性发生逆转 ,但HLA、B7的表达无明显变化。结论 :TAM、VRP可以逆转卵巢癌的MDR细胞 ,敏感株细胞和多药耐药细胞有着不同的免疫逃逸机制 ,与耐药株相比 ,敏感株细胞更易逃避机体的免疫反应 。
Objective:To explore the immune evasion mechanism o f mu ltidrug resistance(MDR)cells in ovarian carcinoma.Methods:Revert an t effects of hydroxytamoxifen(TAM)and verapamil(VRP)on COC1/DDP were assayed by MTT method.Flow cytometry was used to measure the expression of HLA-Ⅰ,HLA-Ⅱ,B7 -1 and B7-2 in COC1 cells and COC1/DDP cell strain before and after MDR reversio n.Resuls:Both COC1/DDP and COC1 cells expressed high HLA-Ⅰ and comparatively lower HLA-Ⅱ,B7-1 and B7-2.However,the expresions of B7-1 and B7-2 antigen in CoC1/DDP cells were higher than that in COC1 cells.With the action o f TAM and VRP,the drug-resistance of COC1/DDP reversed but the expression of HLA and B7 had no change.Conclusion:TAM and VRP can reverse the MDR cells of ovarian carcinoma.Compared with MDR cell strains,it is easier for sens itive cell strains to escape host immune reaction.Adoptive immunotherapy is a po tential choice for the treatment of MDR carcinoma.
出处
《西南国防医药》
CAS
2004年第1期4-6,共3页
Medical Journal of National Defending Forces in Southwest China
