摘要
目的:探讨原因不明复发性流产患者蜕膜组织中Th1/Th2比率与巨噬细胞B7-1、B7-2表达水平的相关性,分析蜕膜组织中共刺激分子B7-1、B7-2及Th1/Th2比率失衡在原因不明复发性流产发病中的作用。方法:用双荧光标记流式细胞技术检测原因不明复发性流产妇女(流产组)和正常妊娠妇女(对照组)的蜕膜组织中Th1/Th2比率和巨噬细胞B7-1、B7-2的表达水平。结果:流产组妇女蜕膜组织中Th1/Th2比率(20.41±3.32)、巨噬细胞B7-1的表达水平(21.57±1.70)%明显高于对照组(P<0.01);流产组妇女蜕膜组织中巨噬细胞B7-2的表达水平(27.82±2.28)%明显低于对照组(P<0.01);流产组妇女蜕膜组织中Th1/Th2比率与蜕膜组织中巨噬细胞B7-1的表达水平之间存在显著正相关(r=0.8663,P<0.01);流产组妇女蜕膜组织中Th1/Th2比率与蜕膜组织中巨噬细胞B7-2的表达水平之间存在显著负相关(r=-0.9016,P<0.01)。结论:原因不明复发性流产患者蜕膜组织中B7-1表达水平增高、B7-2表达水平降低与蜕膜组织中Th1/Th2比率异常有密切的关系,可能是导致原因不明复发性流产发生的重要因素。
Objective： To evaluate the relationship between Th1/Th2 and B7 - 1, B7 -2 in uterine decidua in unexplained recurrent spontaneous abortion （URSA） and to investigate the function of them in the pathogenesis. Methods： The expression of Th1/Th2 and B7 - 1, B7 -2 in uterine decidua from 20 patients with URSA, 19 normal pregnancy women were determined by double - label flow cytometric technique. Results： The expression of Th1/Th2 （20. 41 ＋3. 32） and B7 - 1 （21.57 ± 1.70） % was higher significantly in patients with URSA than that in normal pregnancy women （ P 〈 0. 01 ）. The expression of 137 - 2 （27. 82 ±2.28 ） % was lower significantly in patients with URSA than that in normal pregnancy women （P 〈 0. 01 ）. There were positive correlated between Th1/Th2 and B7 - 1 （ r = 0. 866 3, P 〈 0. 01 ）. There were negative correlated between Th1/Th2 and B7 - 2 （ r = - 0. 901 6, P 〈 0. 01 ） . Conclusion： Overexpression of B7 - 1 and inhibition of 137 - 2 lead to the imbalance of Th1/Th2 , and it might be the reason of URSA.
出处
《中国妇幼保健》
CAS
北大核心
2008年第32期4606-4608,共3页
Maternal and Child Health Care of China
基金
河北医科大学基金资助项目(项目编号:040306)
